Nā huahana

Ua hoʻohana ʻia ʻo 4-Methylumbelliferyl β-D-glucopyranoside ma ke ʻano he substrate: · i ka glucosylceramidase β enzyme activity assay i nā hakina i hoʻonui ʻia i ka lysosome mai nā neurons hippocampal mua · i ka hoʻāʻo β-glucosidase i ka wā fermentation hū · e hōʻike i ka glucocerebrosidase 1 (GBA1) pili. ka hana ma ka laina cell macrophage (RAW)

ʻO 4-Methylumbelliferyl β-D-glucopyranoside kahi substrate enzymatic synthetic no glycosidase.Ua hoʻohana ʻia ma ke ʻano he substrate no ka β-glucosidase mai enterococci.

Hōʻike mākou e hiki i nā mea hāʻawi glycoside maʻalahi ke hoʻololi i ka equilibria o nā hopena glycosyltransferase-catalyzed, e hoʻololi i ka hoʻokumu ʻana o ka NDP-sugar mai kahi endothermic i kahi kaʻina exothermic.No ka hōʻike ʻana i ka pono o kēia thermodynamic adaptability, hōʻike mākou i ka glycosyltransferase-catalyzed synthesis o 22 sugar nucleotides mai nā mea hāʻawi kōpaʻa maʻalahi, a me ka hoʻokumu ʻana i ke kō i loko o nā nucleotides e like me ka ikaika hoʻoikaika i ka pōʻaiapili o ka glycosyltransferase-catalyzed reactions no. liʻiliʻi-mole glycodiversification.ʻO kēia mau mea hāʻawi ʻala maʻalahi i hiki i ka hoʻāʻo colorimetric maʻamau no ka glycosyltransfer, pili i ka loaʻa ʻana o ka lāʻau lapaʻau, ka ʻenekinia protein a me nā noiʻi ʻē aʻe e pili ana i ka nucleotide sugar.Hoʻopiʻi pololei kēia haʻawina i ka manaʻo nui ʻo NDP-sugars he 'kiʻekiʻe-ikaika' hāʻawi kō i ka wā i lawe ʻia mai ko lākou pōʻaiapili kūlohelohe kuʻuna.