4-Nitrophenyl-alpha-D-galactopyranoside CAS:7493-95-0 99%
| Helu Helu | XD900018 |
| inoa mea kūʻai | 4-Nitrophenyl-alpha-D-galactopyranoside |
| CAS | 7493-95-0 |
| ʻĀpana Molekala | C12H15NO8 |
| Kaumaha Molecular | 301.25 |
| Nā kikoʻī mālama | -15 a -20 °C |
| Kānāwai Kūʻai Kūlike | 29400000 |
Huahana Huahana
| Ka nana aku | Keʻokeʻo i ka ʻAlani i ka pauka ʻōmaʻomaʻo i ke aniani |
| Hoʻāʻo | 99% |
ʻO 4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) kahi mea hoʻoheheʻe ʻia o 4-nitrophenyl (pNP) glycopyranoside no ka ʻike ʻana i ka hana α-galactosidase.Ua hoʻonui nui ʻia ka nui o ka pNP i hoʻokuʻu ʻia i ka wā i hoʻohana ʻia ai ka 4-Nitrophenyl α-D-galactopyranoside ma ke ʻano he substrate.
Ke hoʻohana nei i ka 4-nitorophenol (pNP) -glycopyranosidespseudosubstrates, ʻo ka nui o ka pNP hoʻokuʻu ʻia mai ka 4-Nitrophenylα-D-galactopyranoside ʻoi aku ka kiʻekiʻe ma mua o ka mea ʻē aʻe mai ka ʻano ʻē aʻe NP-glycopyranosidesatpH4.0 akā ʻo ka hana ʻole i ʻike ʻia ma kapH7. Hōʻike nā pyranosidesasa artificial substrates i ke koena o ka galactose hoʻokuʻu ʻia mai 4-Nitrophenylα-D-galactopyranoside ma ka nui o kapH4.0, iethepHlevel kahi i emi ai ka paepae i ka nui loa.
Ka lepo o ka α-D-galactosidase (α-D-Galactosidase).Hoʻopaʻa i ka mea lawe pūmua E. coli lactose.Medium no ka α-D-galactose.
Ua hoʻomohala ʻia kahi ala wikiwiki no ka loiloi ʻana i ka hana lytic o nā antimicrobial agents e kūʻē i ka hū a me nā fungi.Hoʻokumu ʻia ka assay ma ka hoʻokuʻu ʻana o ka enzyme intracellular, maltase (alpha-glucosidase).Ua ana ʻia ka hana maltase i hoʻokuʻu ʻia e ka hana ʻana o p-nitrophenol mai p-nitrophenyl-alpha-D-glucopyranoside (PNPG).Ua ana ʻia ka hana lytic o nā pūhui antimicrobial ʻē aʻe e kūʻē i nā pūnaewele hū a i ʻole nā spores e ulu ana o nā haʻi filamentous.Hiki ke ʻike ʻia ka hana anti-yeast Lytic i loko o 20 min incubation ma 30 degere C e kūʻē iā Saccharomyces cerevisiae, Candida albicans, a me Cryptococcus neoformans.Ua ʻike ʻia ka hana anti-fungal Lytic ma hope o 2 h o ka incubation ma 30 degere C e kūʻē i nā spores germinating o Aspergillus niger a me Botrytis cinerea.ʻAʻole i hydrolyze nā pūnaewele holoʻokoʻa o ka hū a i ʻole nā hani i ka PNPG pono i loko o 3 mau hola ma 30 degere C e loaʻa ai kahi hoʻololi kala ʻike ʻia.Ua ʻike ʻia ka hana lytic o nā enzymes (e laʻa, Lyticase), nā lāʻau antibiotic (e laʻa, Amphotericin B), a me kahi maʻi bacteria e hana ana i nā lāʻau antibiotic me ka hoʻohana ʻana i ka hoʻāʻo.Ua hoʻololi ʻia ka hōʻike anti-yeast i kahi ʻano microtiter 96-well.Hāʻawi nā ho'āʻo ʻelua i ka ʻike wikiwiki, paʻakikī, a me ka hana hou ʻana o ka lytic anti-yeast a me ka hana anti-fungal.
