Nā huahana

Hoʻokumu ʻia kahi immunosensor electrochemiluminescence (ECL) ʻole lepili no ka ultrasensitive a me ka wikiwiki o ka maʻi pathogenic bacterium Vibrio parahaemolyticus (VP) i loko o ka wai kai a me ka iʻa kai i hoʻomohala ʻia ma muli o ka graphene oxide multi-functionalized, i hoʻomākaukau ʻia me N-(4-aminobutyl)-N -ethylisoluminol (ABEI) a me VP antibody (anti-VP) i ka manawa like i immobilized ma ka ʻili o ka magnetic graphene oxide (nanoFe3O4@GO).Ua hana ʻo ABEI a me anti-VP ma ke ʻano he electrochemiluminophore a me ka mea hopu no VP.ʻO ka conductivity maikaʻi a me ka hana ʻelua-dimensional o ka nanoFe3O4@GO i hiki ai i ka ABEI āpau, immobilized ma GO, electrochemically active a pēlā i hoʻomaikaʻi ai i ka ʻike ʻike.Ma lalo o nā kūlana maikaʻi loa, ua emi ka ikaika ECL me ka hoʻonui ʻana i ka logarithmic concentrations o VP ma ka laulā o 10-10(8)CFU/mL, me ka palena ʻike o 5CFU/mL no ka wai kai a me 5CFU/g no ka iʻa.Ua hōʻike kēia immunosensor ECL i ka kikoʻī kiʻekiʻe, kūpaʻa a me ka reproducibility no ka ʻike ʻana o VP.Eia kekahi, ua hoʻohana maikaʻi ʻia ka immunosensor ECL e hoʻoholo i ka neʻe ʻana o VP i ka wai kai a me ka iʻa iʻa wikiwiki, me ka hoʻihoʻi ʻana o 94.4-112.0% a me RSD 4.1-11.7%.No laila, hōʻike ka immunosensor i kūkulu ʻia i ka manaʻolana maikaʻi no ka hoʻohana pono ʻana.