Nā huahana

ʻO ka nānā ʻana i ka pharmacodynamics ma kahi o pharmacokinetics kekahi o nā hoʻolālā e hoʻokaʻawale i ka mycophenolate mofetil therapy.ʻO ke kumu o kēia noiʻi ʻana ʻo ia ka hoʻomohala ʻana i nā ʻano kikoʻī chromatography-tandem mass spectrometry (LC-MS/MS) no ka loiloi ʻana i nā pharmacokinetics a me pharmacodynamics o ka mycophenolic acid (MPA).Ua hoʻoholo ʻia ka nui o ka mycophenolic acid glucuronide (MPAG), mycophenolic acid acyl-glucuronide, a me ka unbound MPA a me MPAG, a ua helu ʻia ka hana inosine-5'-monophosphate dehydrogenase ma ke ana ʻana i ka nui o ka xanthosine-5'-monophosphate (XMP) ) a me intracellular adenosine-5'-monophosphate ma hope o ka incubation o nā lysates koko mononuclear cell peripheral (PBMC).Ua hoʻohana ʻia kahi kolamu Mastro(TM) metala ʻole a me ʻelua kumu gradient no ka hoʻomaikaʻi ʻana i ka palena helu o XMP i 0.1 μM.I loko o ke kahua koʻikoʻi o ka MPA, ʻo ka linearity o ka calibration curve, ka pololei a me ka pololei i waena o nā lā i hoʻokō i nā alakaʻi pili i ka US Food and Drug Administration.ʻO ka hoʻopaʻa ʻana o ka MPA i nā maʻi hematopoietic stem cell transplant (HSCT) i hoʻoholo ʻia e ka enzyme assay a me ke ʻano LC-MS/MS o kēia manawa ua hōʻike i kahi pilina maikaʻi (r(2) = 0.95, p <0.001).Ma kēia haʻawina, hōʻike mākou i nā ʻano LC-MS/MS koʻikoʻi a hoʻopaʻa ʻia e loiloi i ka pharmacokinetics a me ka pharmacodynamics o MPA, i lawa pono ke nānā i nā liʻiliʻi liʻiliʻi o nā lysates PBMC i hōʻiliʻili ʻia ma hope koke o ka HSCT.