Nā huahana

ʻO nā hōʻike hou e kākoʻo ana i kahi hana e puka mai ana o ka β-nicotinamide adenine dinucleotide (β-NAD(+)) ma ke ʻano he neurotransmitter novel a me ka neuromodulator i loko o ka ʻōnaehana nerve peripheral -β-NAD(+) i hoʻokuʻu ʻia i loko o ka hoʻomākaukau ʻana i nā ʻiʻo maʻemaʻe a me nā cell chromaffin adrenal. ma kahi ʻano ʻano o kahi neurotransmitter.ʻAʻole maopopo i kēia manawa inā paʻa kēia no ka CNS.Me ka hoʻohana ʻana i kahi ʻano loiloi superfusion liʻiliʻi a me nā ʻenehana chromatography wai kiʻekiʻe, hōʻike mākou i ka hoʻoulu ʻana o ka kiʻekiʻe-K (+) o nā synaptosomes forebrain forebrain e hoʻāla i ka nui o β-NAD (+), adenosine 5'-triphosphate, a me kā lākou metabolites adenosine 5'-diphosphate (ADP), adenosine 5'-monophosphate, adenosine, ADP-ribose (ADPR) a me ADPR cyclic.ʻO ke kiʻekiʻe-K (+) -evoked overflow o β-NAD (+) ua hoʻemi ʻia e ka cleavage o SNAP-25 me ka botulinum neurotoxin A, ma ke kāohi ʻana i nā kahawai N-type voltage-dependent Ca (2+) me ω-conotoxin GVIA , a ma ka pale ʻana i ka gradient proton o nā vesicle synaptic me ka bafilo mycin A1, e hōʻike ana e hoʻokuʻu ʻia ka β-NAD(+) ma o ka vesicle exocytosis.Hōʻike ka ʻike komohana i ka CD38, kahi protein multifunctional e metabolizes β-NAD(+) , aia ma nā membrane synaptosomal a me ka cytosol.Hoʻohaʻahaʻa nā synaptosomes paʻa i ka β-NAD(+) .ʻO 1,N (6) -etheno-NAD, kahi analog fluorescent o β-NAD(+), lawe ʻia e synaptosomes a ua hoʻemi ʻia kēia uptake e ka β-NAD (+) maoli, akā ʻaʻole e ka connexin 43 inhibitor Gap 27. I loko o nā neurons cortical noiʻi kūloko o β-NAD(+) kumu wikiwiki Ca(2+) transients, ma muli paha o ka influx o extracellular Ca(2+).No laila, hiki i nā synaptosomes lolo ʻiole ke hoʻokuʻu ikaika, hoʻohaʻahaʻa a lawe i ka β-NAD(+), a hiki i ka β-NAD(+) ke hoʻoulu i nā neurons postsynaptic, nā pono āpau e pono ai no kahi mea i manaʻo ʻia he neurotransmitter moho i loko o ka lolo.