Nā huahana

Hoʻokumu ʻia nā biofilms me nā cell bacteria i hoʻopaʻa ʻia i loko o kahi matrix polymeric extracellular i hana ponoʻī.ʻO ka Poly-beta(1,6)-N-acetyl-d-glucosamine (PNAG) kahi mea nui biofilm matrix matrix i loko o ka phylogenetically diverse bacteria.Ma kēia haʻawina ua noiʻi mākou i nā waiwai kino a me nā kemika o ka PNAG matrix i nā biofilms i hana ʻia i loko o ka vitro e ka gram-negative porcine respiratory pathogen Actinobacillus pleuropneumoniae a me ka gram-positive device-pili pathogen Staphylococcus epidermidis.Ua hoʻoholo ʻia ka hopena o ka PNAG ma ke kahe ʻana o ka wai nui ma ke ana ʻana i ka nui o ka convection wai ma o nā biofilms moʻomeheu i nā mea kānana centrifugal.Ua ʻoi aku ka kiʻekiʻe o ka nui o ka convection wai i loko o nā biofilms moʻomeheu i mua o ka PNAG-degrading enzyme dispersin B ma mua o nā biofilms moʻomeheu me ka ʻole o ka enzyme, e hōʻike ana e hoʻemi ka PNAG i ka kahe wai nui.Ua ālai pū ʻo PNAG i ka lawe ʻana o ka quaternary ammonium compound cetylpyridinium chloride (CPC) ma o nā biofilms.ʻO ka hoʻopaʻa ʻana i ka CPC i nā biofilms ua keakea hou aku i ka hoʻoheheʻe ʻana i ka wai a ua ālai ʻia ka lawe ʻana o ka azo dye Allura ʻulaʻula.Ua hoʻokaʻawale maikaʻi ʻia ʻo Bioactive CPC mai nā biofilms e ka mālama ʻana me 1 M sodium chloride.Hoʻohui pū ʻia, hōʻike kēia mau ʻike e hana pololei ʻo CPC me ka matrix PNAG a hoʻololi i kāna mau waiwai kino a me ke kemika.Hōʻike kā mākou mau hopena he hana koʻikoʻi ka PNAG i ka mālama ʻana i ke kūlana physiological o nā biofilms a hiki ke kōkua i nā kaʻina hana pili biofilm e like me ke kū'ē biocide.