Nā huahana

ʻO kahi ala lapaʻau koʻikoʻi no ka mālama ʻana i ka maʻi maʻi type 2 ʻo ia ka hoʻohana ʻana i nā mea hana e hiki ke hoʻemi i ka hyperglycaemia postprandial ma ke kāohi ʻana i nā enzyme e hoʻoheheʻe ana i ka carbohydrate.Ua noiʻi ka haʻawina i kēia manawa i nā hopena o ka bioassay-guided extract a me nā hapa o ka pericarp hua maloʻo o Phaleria macrocarpa, kahi mea kanu anti-diabetic maʻamau, ma ka α-glucosidase a me ka α-amylase, i mea e hoʻomaopopo ai i kā lākou hana anti-diabetic. Methanol extract (ME), loaʻa ma ka hoʻoheheʻe ʻana i nā mea hoʻoheheʻe, ua loiloi ʻia kona ʻāpana wai-wai n-butanol (NBF) a me ke kolamu flash chromatographic sub-fraction (SFI). no ka mea in vitro α-glucosidase (yeast) a me α-amylase (porcine) hana inhibition.Eia kekahi, ua hana ʻia nā hōʻike hōʻoia i loko o ka vivo i nā ʻiole maʻi maʻi streptozotocin-induced (SDRs) me ka hoʻohana ʻana i ka glucose waha, sucrose a me ka starch tolerance tests. 75%) a me α-amylase (87%) i loko o ka vitro (IC50 = 2.40 ± 0.23 μg / ml a me 58.50 ± 0.13 μg / ml, kēlā me kēia) i loko o keʻano o ka hopena;ʻO kahi hopena i ʻike ʻia ma kahi o 20% kiʻekiʻe ma mua o ka acarbose (55%), kahi mea paʻa α-glucosidase maʻamau (IC50 = 3.45 ± 0.19 μg/ml).Ua kāohi pū ka ME a me SFI i ka α-glucosidase (IC50 = 7.50 ± 0.15 μg / ml a me 11.45 ± 0.28 μg / ml) a me ka α-amylase (IC50 = 43.90 ± 0.19 μg / ml a me 69.80 μg / ml) liʻiliʻi liʻiliʻi.I loko o nā haʻawina in vivo me nā ʻiole diabetic, ʻo ka NBF a me ka SFI i hōʻemi maikaʻi i ka glucose koko kiʻekiʻe (PBG) e 15.08% a me 6.46%, a me ka wahi ma lalo o ka curve tolerance (AUC) e 14.23% a me 12.46%, kēlā me kēia, ma hope o kahi hoʻokūkū o ka sucrose waha. (P <0.05);ma laila e hōʻoia ai i ka hana in vitro i ʻike ʻia.Ua hōʻike pū ʻia kēia mau hopena hōʻemi i ka PBG a me AUC i nā hoʻāʻo ʻana i ka glucose a me ka starch tolerance, akā i kahi pae liʻiliʻi. he mea kanu kūpono no ka ʻimi ʻana i nā pūhui kūlohelohe no ka mālama ʻana i ka maʻi diabetes type 2.