Nā huahana

ʻO nā maʻi Uropathogenic o Escherichia coli (UPEC) ke kumu nui o nā maʻi maʻi urinary tract.Hoʻopili ʻia ke ola i loko o ke kahe koko me nā ʻano hana like ʻole e kōkua i ke kūʻē ʻana i ka serum complement-mediated pepehi.ʻOiai ua hōʻike ʻia ka heterogeneity phenotypic o ka bacteria e hoʻoikaika i ka hoʻomanawanui antibiotic, ʻaʻole i hoʻāʻo ʻia ka hiki ke hana i nā cell refractory i ka pepehi ʻana e ka ʻōnaehana pale.Ma ka noiʻi ʻana i kēia manawa ua ʻimi mākou e hoʻoholo inā pili ka heterogeneity o nā moʻomeheu bacteria i ka huli ʻana i ka bacteria e ka ʻōnaehana serum complement.Ua nānā mākou i nā ʻāpana cell i ka UPEC strain CFT073 me ka protein reporter fluorescent.Hoʻokomo ʻia nā pūnaewele stationary-phase i loko o ka serum kanaka ikaika a i ʻole ka wela i ke alo a i ʻole ka loaʻa ʻole o nā lāʻau ʻokoʻa (ampicillin, norfloxacin, a me amikacin), a ua ana ʻia ka māhele cell a me ka hoʻopili ʻana i ka protein C3 e ka kahe cytometry a me ka microscopy immunofluorescence.ʻO ka heterogeneity i nā manawa pālua o CFT073 pūnaewele i loko o ka serum i hiki ke hoʻokaʻawale ʻia i ʻekolu subpopulations phenotypically like ʻole, ʻike ʻia lākou āpau e ka ʻāpana C3 o ka ʻōnaehana hoʻopihapiha.ʻO ka heluna kanaka o ka ulu wikiwiki ʻana e kūʻē i ka serum complement-mediated lysis.ʻO ka subpopulation nui o nā pūnaewele me ka ulu ulu waena e maʻalahi i ka serum.ʻO ke kolu o ka heluna kanaka, ʻaʻole e hoʻomaka hou i ka ulu ʻana ma ka hoʻoheheʻe ʻana mai ka manawa paʻa, pale ʻia mai ka serum complement a me nā antibiotics.