Nā huahana

Introduction: ʻO BSA kekahi o nā polokina i hoʻohana mau ʻia i loko o nā keʻena hana biochemical, a hiki ke nānā ʻia kona koʻikoʻi i nā hoʻokolohua no ka mea maʻamau a maʻamau loa.ʻO Bovine serum albumin (BSA), i ʻike ʻia ʻo ka ʻāpana ʻelima, he globulin i loko o ka serum bovine i loaʻa nā koena amino acid 583, me ke kaumaha molekala o 66.430kDa a me kahi helu isoelectric o 4.7.Loaʻa i ka BSA ka nui o nā noi i nā hoʻokolohua biochemical, e like me kahi mea pale pale i nā blots komohana.

Noi: ʻO Bovine Serum Albumin (BSA), i ʻike ʻia ʻo ka ʻāpana ʻelima, he globulin i loko o ka bovine serum, nona nā koena amino acid 607, a he nui nā noi i nā hoʻokolohua biochemical.Hoʻohana pinepine ʻia ʻo Bovine serum albumin ma ke ʻano he stabilizer i ka hopena mālama a me ka hopena hopena o nā enzyme restriction a i ʻole nā ​​enzyme i hoʻololi ʻia e hoʻopaʻa i ka hana enzyme a pale i ka decomposition enzyme a me ka adsorption non-specific.

Hana: Hoʻohana maʻamau ʻo BSA ma ke ʻano he mea hoʻopaʻa i loko o ka hoʻopaʻa ʻana a me ka hoʻonā hopena o nā enzyme kaohi a i ʻole nā ​​enzyme i hoʻololi ʻia, no ka mea, paʻa ʻole kekahi mau enzymes a haʻahaʻa paha ka hana ma nā haʻahaʻa haʻahaʻa.Ma hope o ka hoʻohui ʻana i ka BSA, hiki iā ia ke hana i ke kuleana o ka "pale" a i ʻole "mea lawe", a hiki ke hoʻomaikaʻi maikaʻi ʻia ka hana o nā enzyme he nui ma hope o ka hoʻohui ʻana i ka BSA.ʻAʻole pili ʻia nā enzyme i koi ʻole i ka hoʻohui ʻana o BSA e ka hoʻohui ʻana o BSA.No ka hapa nui o ka DNA substrate, hiki i ka BSCA ke hoʻopiha i ka hoʻoheheʻe ʻana, a hiki ke hoʻokō i ka ʻoki pinepine ʻana.Ma 37 ° C, i ka wā i ʻoi aku ai ka hopena o ka digestion ma mua o 1 h, hiki i ka BSA ke hoʻoikaika i ka enzyme, no ka mea, i loko o ka buffer pane me ka ʻole o BSA, hiki i nā enzyme kaohi ke ola wale ma 37 ° C no 10 ~ 20min a i ʻole ka manawa pōkole..Ma ka ʻokoʻa, hiki i ka BSA ke hoʻopaʻa i nā ion metala a me nā mea ʻē aʻe i loko o ka buffer a i ʻole substrate DNA e kāohi ai i ka hana o nā endonucleases palena.

Hoʻohana: Standard grade bovine serum albumin (BSA, StandardGrade), hiki ke hoʻokō i nā pono o ka hapa nui o nā hoʻokolohua maʻamau, e like me ka immunoblocking agent, tissue cell (microbial animal and insect cell, etc.) moʻomeheu moʻomeheu a me nā mea moʻomeheu, protein/enzyme stabilization nā reagents a me nā kūlana quantification protein.Hiki i ka Diagnostic grade bovine serum albumin (BSA, DiagnosticGrade) ke hoʻokō i nā pono hoʻokolohua maʻamau, e like me ka immunoblocking agent, protein/enzyme stabilizer, diluent, carrier and protein standard.Eia kekahi, hiki ke hoʻohana ʻia no ka immunoassays, ka moʻomeheu cell a me nā hoʻokolohua hybridization e koi ai i ka ʻike kiʻekiʻe.