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Hoʻopili kēia haʻawina i ka gene alanine racemase (alr-2), i komo i ka synthesis o d-alanine i hana i ka iwi kuamoʻo o ka paia o ke kelepona.Ua kūkulu ʻia kahi mutant stable alr-2 knockout o Aeromonas hydrophila HBNUAh01.I ka hoʻohui ʻia ʻana o ka mutant me ka d-alanine, ʻaʻole i hoʻopilikia ʻia ka ulu ʻana;ʻO ka nele o ka d-alanine ka mea i hoʻopaʻa ʻia ka ulu ʻana o nā pūnaewele mutant pōloli, akā ʻaʻole ka cell lysis.ʻAʻohe hana alanine racemase i ʻike ʻia ma ka moʻomeheu o ka mutant.Eia hou, ua hōʻike ʻia kahi hoʻokolohua membrane permeability i ka hoʻonui ʻana i ka pōʻino i ka paia o ke kelepona i ka wā pololi d-alanine.ʻAʻole i ʻike ʻia kēlā ʻano pōʻino i ke ʻano hihiu i ka wā moʻomeheu.ʻO ka nānā ʻana a me ka hoʻoili ʻana i ka microscopy electron i hōʻike ʻia i nā hemahema o ka envelopp cell a me ka perforation o ka paia cell.Ua ʻike ʻia ka leaka ʻana o nā mea e hoʻopaʻa ai i ka UV mai nā mutants.No laila, ʻo ka hiki ʻana o nā mutants a me ko lākou kūʻokoʻa o d-alanine no ka ulu ʻana i hōʻike ʻia ʻaʻole i hoʻokau ʻia ka inactivation o alr-2 i kahi koi auxo trophic no d-alanine.© FEMS 2015. Aia nā kuleana a pau.