L-Alanine CAS: 56-41-7 99% Keʻokeʻo crystalline pauka
| Helu Helu | XD90326 |
| inoa mea kūʻai | D-Alanine |
| CAS | 338-69-2 |
| ʻĀpana Molekala | C3H7NO2 |
| Kaumaha Molecular | 89.09 |
| Nā kikoʻī mālama | Ambient |
| Kānāwai Kūʻai Kūlike | 29224985 |
Huahana Huahana
| Ka nana aku | ʻO ka pauka kristal keʻokeʻo |
| Hoʻāʻo | 98.5 - 101.5% |
| Kahuli kiko'ī | +13.7 a i +15.1 |
| Nā metala kaumaha | <0.0015% |
| pH | 5.5 - 7 |
| SO4 | <0.03% |
| Nalo ma ka maloo | <0.2% |
| hao | <0.003% |
| Koena ma ka Ignition | <0.15% |
| Ka nui o ka ʻāpana | 200um |
| Cl | <0.05% |
| Ka pae o nā mea hoʻohaumia organik mau | ʻAʻohe mea haumia |
Hoʻokaʻawale ʻo γ-Glutamyltranspeptidases (γ-GTs) i ka paʻa γ-glutamyl amide o ka glutathione a hoʻololi i ka hui γ-glutamyl i hoʻokuʻu ʻia i ka wai (hydrolysis) a i ʻole nā ʻakika amino acceptor (transpeptidation).ʻO kēia mau enzymes ubiquitous ke kuleana nui i ka biosynthesis a me ka degradation o glutathione, a me ka detoxification xenobiotic.Maʻaneʻi mākou e hōʻike nei i ka 3Å hoʻonā aniani hoʻonā o Bacillus licheniformis γ-GT (BlGT) a me kona paʻakikī me l-Glu.Hōʻoia nā hale X-ray e pili ana ʻo BlGT i ka N-terminal nucleophilic hydrolase superfamily a hōʻike ʻia aia ka pūmua i ka wehe ʻana o ka wahi hana e like me ka mea i hōʻike ʻia no ka enzyme homologous mai Bacillus subtilis, akā ʻokoʻa i nā mea i ʻike ʻia no ke kanaka γ-GT a no nā γ-GT mai nā microorganism ʻē aʻe.Hōʻike ka ʻikepili i ka hoʻopaʻa ʻana o ka l-Glu i ka hoʻonohonoho hou ʻana o ka huelo C-terminal o BlGT subunit nui a ʻae i ka ʻike ʻana i kahi pūʻulu o nā koena waikawa i komo i ka ʻike ʻana i kahi ion metala.Ua aʻo ʻia ke kuleana o kēia mau koena ma ka conformational stability o BlGT ma ke ʻano o ka autoprocessing, hana enzymatic, kemika a me ka thermal denaturation o ʻehā mau mutants Ala hou.Hōʻike nā hopena i ka hoʻololi ʻana o Asp568 me kahi Ala e pili ana i ka autoprocessing a me ka paʻa o ka protein.
