Nā huahana

ʻAʻole ʻike maikaʻi ʻia nā ala molekika pathogenic i nā maʻi ate cirrhotic e like me ka maʻi hepatitis C (HCV), ka maʻi hepatitis autoimmune (AIH) a me ka cirrhosis biliary mua (PBC).He mea koʻikoʻi nā genes i hōʻike ʻia i ka maʻi pathogenesis.ʻO ka suppression subtractive hybridization (SSH) kahi ala genome-ākea e hoʻonui ai i nā transcripts mRNA hōʻike ʻokoʻa.Ua manaʻo mākou e hana i nā ʻike hou o ka hōʻike ʻana o ke ʻano ma ka cirrhosis me ka hoʻohana ʻana iā SSH i hui pū ʻia me ka quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR).Ua nānā ʻia nā transcriptomes ate i ka HCV cirrhosis, AIH cirrhosis, PBC, a me ka ʻiʻo ate nondiseased e SSH.Hoʻopili hou ʻia nā clones DNA hoʻohui (cDNA) no ka hōʻike ʻokoʻa e ka dot-blot hybridization a laila hoʻonohonoho ʻia.Ua helu ʻia ka ʻōlelo gene i koho ʻia e ka RT-PCR manawa maoli.Ma hope o SSH, ua nānā hou ʻia nā clones 694 no ka hōʻike ʻana i nā ʻano genes, a ʻo 145 i hoʻonohonoho ʻia a loaʻa mai nā ʻano genes like ʻole 89.He homologous wale nō nā clones ʻehiku me nā kaʻina hoʻololi i hōʻike ʻia (EST) i nā genes me ka ʻike ʻole o ka hana.Ua hōʻoia ʻia ka hōʻike ʻana i luna o nā genes ʻehā e ka RT-PCR manawa maoli: transmembrane 4 superfamily member 3 (tetraspanin CO-029) i nā ʻano āpau o ka cirrhosis, hedgehog interacting protein (HIP) ma AIH cirrhosis a me chitinase 3-like-1 (HC gp-39 a i ʻole ykl-40) a me ka arginine-glutamic acid hou (RERE) i ka HCV cirrhosis.Ua ʻike ʻia ka RERE gene polymorphisms a me nā ʻano ʻano splice ma nā ʻiʻo āpau i nānā ʻia.ʻO Tetraspanin CO-029 up-regulation i hoʻonohonoho mua ʻia i nā cell ductular bile.I ka hopena, ua hana ʻia nā ʻike hou o ka hōʻike ʻokoʻa gene i ka cirrhosis kanaka me ka hoʻohana ʻana iā SSH ma ke ʻano he mea ʻike mua.ʻO ka mea nui, pono nā haʻawina hou o ka RERE a me kāna mau huahana i ka maʻi ate pili i ka HCV.