Nā huahana

Hāʻawi ka hyperpermeability vascular i ka morbidity i ka mumū.ʻO nā ʻano hana i kēia manawa no ka loiloi in vivo o ka permeability e pili ana i ka extravasation o Evans Blue (EB)-bound albumin he paʻakikī a pinepine ʻole i ka naʻau.Ua hoʻomohala mākou i kahi ʻano hana hou infrared fluorescence (IRF) no ke ana ʻana i ka extravasation EB-albumin e helu i ka permeability vascular i nā hiʻohiʻona murine.Ua nānā ʻia ka ʻili a me ka peritoneum e ka IRF a me ke ana ʻana o ka EB ma ke ʻano o ka ʻiʻo.Ua hoʻonui ʻia ka IRF o ke kino me ka piʻi ʻana o ka nui o ka EB intravenous (2.5-25 mg/kg).Ua ʻoi aku ka maʻalahi o ka Tissue IRF no ka hōʻiliʻili ʻana o EB i hoʻohālikelike ʻia i ke ʻano hana hoʻopalekana.No laila, ʻokoʻa ka ʻokoʻa o ka vascular permeability a me ka hōʻiliʻili ʻana o ke kino EB ma waena o nā ʻiole i mālama ʻia i ka lipopolysaccharide a me nā ʻiole i mālama ʻia i ka paʻakai i ka wā i kālailai ʻia e ka ʻike ma muli o ka IRF akā ʻaʻole ma ka ʻike ma muli o ka absorbance.Ua ʻike ʻia ʻo EB ma nā ʻāpana 353 a pau i kālai ʻia me IRF akā i loko o 67% (239/353) wale nō o nā ʻōkana i kālai ʻia e ke ʻano o ka absorbance-based, e hōʻike ana i ka hoʻomaikaʻi ʻana i ka ʻike ʻana o ka EB i loko o nā kino me IRF.I ka hoʻokaʻawale, EB i loko o ka plasma ma hope o ka hoʻokele EB ua ana maʻalahi ʻia e nā ʻano ʻelua me ka pilina kiʻekiʻe ma waena o nā ʻano ʻelua (n=116, r2=0.86).ʻOi aku ka maikaʻi o ka helu ʻana o nā ʻokoʻa EB-IRF ma muli o ka endotoxin i ka wā i hoʻohālikelike ʻia ka IRF ma waena o nā ʻiole i hoʻohālikelike ʻia no ke kaumaha, ke kāne, a me ka makahiki, a me nā hoʻoponopono kūpono no ke kaumaha o ke kino a me ka EB plasma concentrations.ʻO ka mea nui, waiho ʻia nā ʻano hana EB-IRF i nā kino no ka histopathology ma hope.I ka hōʻuluʻulu manaʻo, ʻo EB-IRF kahi moʻolelo, koʻikoʻi koʻikoʻi, wikiwiki, a maʻalahi hoʻi no ka helu ʻana o ka EB i loko o nā kino paʻa o ka mālama ʻana me nā ʻiole mana.