Nā huahana

Ua hoʻomohala mākou i kahi ʻōnaehana hoʻopuka liʻiliʻi liʻiliʻi RNA (siRNA) me ka hoʻohana ʻana i kahi paʻakikī ternary me ka polyethyleneimine (PEI) a me ka γ-polyglutamic acid (γ-PGA), i hōʻike i ka hopena silencing a ʻaʻohe cytotoxicity.ʻO nā hui binary o siRNA me ka PEI he 73-102 nm ka nui o ka nui a me ka 45-52 mV i ka ζ-hiki.Ua hoʻonui ʻia ka hopena silencing o siRNA/PEI complexes me ka piʻi ʻana o ka PEI, a ʻo nā complexes siRNA/PEI me ka lākiō uku ʻoi aku ka nui ma mua o 16 i hōʻike i ka luciferase knockdown nui i loko o kahi kiʻi colon carcinoma cell line e hōʻike mau ana i ka luciferase (Colon26/Luc cell).Eia nō naʻe, ua ʻike ʻia ka cytotoxicity ikaika a me ka agglutination koko i loko o ka complex siRNA / Lipofectamine a me siRNA / PEI16 complex.ʻO ka hoʻouka hou ʻana i nā complexes cationic me kahi pūhui anionic i hōʻike ʻia he ala hoʻohiki no ka lanakila ʻana i kēia mau mea ʻona.No laila mākou i hoʻomākaukau ai i nā paʻakikī ternary o siRNA me ka PEI (ka uku hoʻoili 16) ma o ka hoʻohui ʻana o γ-PGA e hōʻemi i ka cytotoxicity a hāʻawi i ka siRNA.E like me ka mea i manaʻo ʻia, ua emi ka cytotoxicity o nā complexes ternary me ka hoʻonui ʻana o ka maʻiʻo γ-PGA, kahi i hoʻemi ai i ka ζ-hiki o nā paʻakikī.Ua ʻike ʻia kahi hopena silencing ikaika i hoʻohālikelike ʻia me siRNA/Lipofectamine complex i nā paʻakikī ternary me ka γ-PGA me kahi uku anionic surface charge.Ua hoʻopaʻa ʻia ka hoʻohui kiʻekiʻe o nā paʻakikī ternary i loko o Colon26/Luc cell me ka microcopy fluorescence.I ka loaʻa ʻana o ka knockdown o kahi gene exogenously transfected, hiki i ka paʻakikī ke hoʻoponopono i ka knockdown o kahi ʻano mālama hale endogenous, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), ua loiloi ʻia i loko o nā pūnaewele B16-F10.ʻO ka paʻakikī ternary (siRNA / PEI16 / γ-PGA12 complex) i hōʻike i kahi hopena GAPDH knockdown koʻikoʻi.No laila, ua hoʻomohala mākou i kahi ʻōnaehana lawe siRNA pono.