Nā huahana

ʻO BBS4 kekahi o nā protein nona nā hemahema ke kumu o Bardet-Biedl Syndrome (BBS), he maʻi maʻi multi-systemic, e hōʻike ana me ka momona nui.Ua hoʻopili ʻia nā polymorphisms BBS4 me ka momona maʻamau non-syndromic morbid.ʻAʻole ʻike piha ʻia nā ʻano molecular obesity BBS4, a me ke kuleana o ka gene BBS4 i ka ʻokoʻa adipocyte a me ka hana.Hōʻike mākou i kēia manawa he kuleana kūpono a koʻikoʻi ʻo Bbs4 i ka proliferation a me adipogenesis: silencing of Bbs4 in 3T3F442A preadipocytes induced accelerated cell division and aberrant differentiation, ʻike ʻia ma o nā haʻawina morphologic (mālamalama, scanning a me ka hoʻouna ʻana i ka microscopy electron), metabolic analysis (ka momona momona, fatty acid profile and lipolysis) a me nā transcripts markers adipogenic (Cebpα, Pparγ, aP2, ADRP, Perilipin).I loko o ka adipogenesis a i ka wā e hoʻopiʻi ʻia ai me ka ukana momona, ʻoi aku ka nui o nā triglycerides i hoʻopaʻa ʻia ma Bbs4 ma mua o ka mana adipocytes, ʻoiai i loko o nā kulu liʻiliʻi (ʻoi aku ka nui o ka helu) i loaʻa i nā profiles fatty acid i hoʻololi ʻia.No laila, ʻoi aku ka nui o ka momona i loko o nā keena i hoʻopaʻa ʻia ma muli o ka piʻi ʻana o ka nui o ka adipocyte proliferation a me ka ʻokoʻa aberrant e alakaʻi ana i ka hōʻiliʻili aberrant o ka momona i kēlā me kēia cell.Hōʻike kā mākou mau ʻike e pili ana ka momona o ka BBS ma muli o ke kuleana pololei o BBS4 i nā hana physiological a me nā pathophysiological e hoʻokumu i ka hoʻokumu ʻana o nā kiko adipose e pili ana i ka momona.