L-Cystine CAS: 56-89-3 99% Keʻokeʻo keʻokeʻo a i ʻole ka pauka crystalline
| Helu Helu | XD90322 |
| inoa mea kūʻai | L-Cystine |
| CAS | 56-89-3 |
| ʻĀpana Molekala | C6H12N2O4S2 |
| Kaumaha Molecular | 240.30 |
| Nā kikoʻī mālama | Ambient |
| Kānāwai Kūʻai Kūlike | 29309013 |
Huahana Huahana
| Ka nana aku | ʻO nā kristal keʻokeʻo a i ʻole ka pauka crystalline |
| Hoʻāʻo | 99% |
| Papa | USP32 |
| Kahuli kiko'ī | -215° a i -225° |
| Nā metala kaumaha | <0.0015% |
| AS | 1.5ppm max |
| SO4 | 0.040% ka nui |
| Fe | <0.003% |
| Nalo ma ka maloo | 0.20% ka nui |
| Koe ma ka Ignition | 0.10% ka nui |
| Cl | 0.10% ka nui |
ʻO nā hale aniani o ka carboxypeptidase T (CpT) paʻakikī me ka phenylalanine a me ka arginine substrate analogs - benzylsuccinic acid a me (2-guanidinoethylmercapto) succinic acid - ua hoʻoholo ʻia e ke ʻano hoʻololi molekala ma nā ʻōlelo hoʻonā o 1.57 Å a me 1.62 Å e hoʻomaʻamaʻa i ka ʻike kikoʻī o ka substrate. o ka enzyme.Ua hōʻike ʻia nā koena conservative Leu211 a me Leu254 (ʻo ia hoʻi i loko o ka carboxypeptidase A a me ka carboxypeptidase B) he mau mea hoʻoholo no ka ʻike ʻana i nā substrates hydrophobic, ʻoiai ʻo Asp263 no ka ʻike ʻana i nā substrates i hoʻopiʻi maikaʻi ʻia.Hoʻololi ka hoʻololi ʻana o kēia mau mea hoʻoholo i ka profile substrate: loaʻa i ka ʻano CpT Leu211Gln nā waiwai e like me carboxypeptidase B, a ʻo ka ʻano CpT Asp263Asn ka carboxypeptidase A-like selectivity.Ua hōʻike ʻia ke kuleana o ka loop Pro248-Asp258 e pili ana me Leu254 a me Tyr255 no ka ʻike ʻana i ke koena C-terminal o ka substrate.ʻO ka hoʻopaʻa ʻana i ka substrate ma ka subsite S1 'alakaʻi i ka hoʻololi ligand-dep endent o kēia loop, a ʻo ka neʻe ʻana o ke kaulahao ʻaoʻao Leu254 e hoʻoulu i ka hoʻonohonoho hou ʻana o ke koena Glu277 koʻikoʻi no ka catalysis.He ʻike hou kēia i ke koho substrate o nā metallocarboxypeptidases e hōʻike ana i ke koʻikoʻi o nā pilina ma waena o ka S1 'subsite a me ke kikowaena catalytic.
