Nā huahana

ʻO ke koʻikoʻi o ka pale ʻana i ka microbial i hui pū ʻia me ka nele o nā antimicrobials hou ua hoʻonui i ka hoihoi i ka hoʻomohala ʻana i nā peptides antimicrobial (AMPs) ma ke ʻano he therapeutics novel.Ma kēia haʻawina, ua loiloi mākou i nā hana antimicrobial o ʻelua peptides synthetic pōkole, ʻo ia hoʻi, RRIKA a me RR.Ua hōʻike ʻia kēia mau peptides i ka hana antimicrobial ikaika e kūʻē iā Staphylococcus aureus, a ua hoʻonui nui ʻia ko lākou mau hopena antimicrobial ma ka hoʻohui ʻana i ʻekolu amino acids i loko o ka C terminus, a no laila ua hoʻonui i ka amphipathicity, hydrophobicity, a me ka uku ʻupena.Eia kekahi, ua hōʻike ʻo RRIKA a me RR i kahi hopena bactericidal koʻikoʻi a wikiwiki hoʻi e kūʻē i nā mea hoʻokaʻawale Staphylococcus lapaʻau a me nā lāʻau lapaʻau, me ka methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-intermediate S. aureus (VISA), vancomycin-resistant S. aureus (VRSA) , Linezolid-resistant S. aureus, a me ka methicillin-resistant Staphylococcus epidermidis.Ma ka hoʻohālikelike ʻana me nā AMP kūlohelohe he nui, ua mālama ʻo RRIKA a me RR i kā lākou hana i mua o nā ʻano physiological o NaCl a me MgCl2.Ua hoʻonui ʻo RRIKA a me RR i ka pepehi ʻana i ka lysostaphin ma mua o 1,000-fold a hoʻopau i ka MRSA a me VRSA kaawale i loko o 20 min.Eia kekahi, ʻoi aku ka maikaʻi o nā peptides i hōʻike ʻia i ka hōʻemi ʻana i nā biofilm adherent o S. aureus a me S. epidermidis i hoʻohālikelike ʻia me nā hopena me nā lāʻau antibiotic maʻamau.Hōʻike kā mākou ʻike i ka hopena staphylocidal o kā mākou peptides ma o ka permeabilization o ka membrane bacteria, e alakaʻi ana i ka leakage o nā mea cytoplasmic a me ka make cell.Eia kekahi, ʻaʻole ʻona nā peptides i nā pūnaewele HeLa ma 4- a i 8-fold ko lākou mau antimicrobial concentrations.ʻO nā hana antimicrobial ikaika a paʻakai ʻole o kēia mau peptides e hōʻike i kahi moho hoʻōla maikaʻi no ka mālama ʻana i nā maʻi S. aureus kūʻē i nā lāʻau he nui.