Nā huahana

ʻO ka biotransformation o methyl-β-D-glucopyranoside i nā kaulahao alkyl glucosides kiʻekiʻe e ka cell bound β-glucosidase o Pichia etchellsii.

Ma ka haʻawina o kēia manawa, ua noiʻi mākou i ka hoʻohana ʻana o Pichia etchellsii nā pūnaewele holoʻokoʻa no ka synthesis o nā alkyl glucosides lōʻihi.Ua hoʻohana ʻia ka Methyl-β-d-glucopyranoside (MG) i ka pane ʻana me nā ʻawaʻawa momona, n-hexanol, n-octanol, n-decanol a me n-dodecanol e synthesize i nā alkyl glucosides.ʻO nā kūlana hoʻonā mua i hoʻopaʻa mua ʻia ma ka pālākiō 2.5 ml no ka synthesis o ka octyl glucoside (OG) a he 8% ka maʻi wai, 100mM MG a me 6h o ka manawa pane a loaʻa kēia i ≈ 53% hua.Ua loaʻa ka nui o ka transglucosylation/hydrolysis ratio o 2.79 ma 100mM MG e makemake ana i ka huahana kiʻekiʻe.Ma muli o nā kūlana i hoʻopaʻa ʻia, ua hoʻohana ʻia kahi reactor ma 50 ml pae i hopena i ≈ 60% hoʻololi o MG i OG.Ua hoʻomohala ʻia kahi ʻano hana chromatography wai maʻalahi no ka helu ʻana o nā kaulahao kiʻekiʻe me ka hoʻohana ʻana i kahi mea ʻike kuhikuhi refractive.Loaʻa ka nui o 27% a me 13% hua no ka decyl-, a me ka dodecyl-β-d-glucopyranoside, kēlā me kēia.