Nā huahana

Ua noiʻi ʻia ka hoʻopaʻa ʻana o ka alizarin melemele G--he derivative azo o ka salicylic acid--e ka bovine serum albumin me ka hoʻohana ʻana i ke ʻano o ka equilibrium dialysis.Ua ʻike ʻia he ʻeono mau wahi paʻa ikaika a me nā mea hou aʻe, nāwaliwali.Hōʻike ʻia ka ʻōnaehana e ka hui like maikaʻi ma waena o nā pūnaewele mua a me ka lua.Ua ho'oholo 'ia nā mea mau e pa'a 'eono ma ke kumu ho'ohālike makemakika ma'alahi.Ma kahi o 2 X 10(4) M-1 nā hualoaʻa no ka paena paʻa mua, 6 X 10(5) M-1 no ka lua, a ma waena o 4 X 10(4) a me 10(5) M-1 no ka hoomaha.Kūkākūkā ʻia ka hanana e pili ana i ka noho ʻana o nā conformers like ʻole a i ʻole ka conformational adaptability o albumin.ʻO ka hui pū ʻana me ka waika salicylic ʻaʻole e hoʻololi i ka alizarin melemele G akā hoʻoulu i kahi hoʻololi conformational i loko o ka protein e pili ana i ka spectrum absorption o ka wai paʻa.E like me ka mea i manaʻo ʻia no kēia ʻano pilina heterotropic, ua like ke ʻano o ka ʻōnaehana albumin-salicylic acid e ka hui pū ʻana o alizarin yellow G.