Nā huahana

Hoʻohana ka nucleoside adenosine i nā ʻōnaehana makaʻu a me ka naʻau ma o ka A2A receptor (A2AR).I ka pane ʻana i ka pae o ka oxygen i loko o nā ʻiʻo, ua hoʻoponopono ʻia ka neʻe ʻana o ka plasma adenosine ma o kāna synthesis e CD73 a ma o kona hoʻohaʻahaʻa ʻia e adenosine deaminase (ADA).ʻO ka cell-surface endopeptidase CD26 e hoʻomalu i ka neʻe ʻana o nā peptides vasoactive a me antioxidant a no laila e hoʻoponopono ai i ka hāʻawi ʻana i ka oxygen i nā ʻiʻo a me ka pane o ke kaumaha oxidative.ʻOiai ua kākau maikaʻi ʻia ka overexpression o adenosine, CD73, ADA, A2AR, a me CD26 i ka pane ʻana i ka hypoxia, pono e noʻonoʻo hou nā hopena o nā kūlana hyperoxic a hyperbaric i kēia mau mea.ʻO nāʻiole a me kahi laina pūnaewele Chem-3 murine e hōʻike ana i ka A2AR i hōʻikeʻia i ka 0.21 bar O2, 0.79 bar N2 (nā kūlana honua; normoxia);1 pahu O2 (hyperoxia);2 bar O2 (hyperbaric hyperoxia);0.21 bar O2, 1.79 bar N2 (hyperbaria).Adenosine plasma concentration, CD73, ADA, A2AR expression, a me ka hana CD26 i kamaʻilio ʻia ma vivo, a ua ʻōlelo ʻia ka hana cAMP i cellulo.No nā kūlana vivo, 1) ua hoʻemi ka hyperoxia i ka pae adenosine plasma a me ka hana CD26 cell T cell surface, akā ua hoʻonui i ka hōʻike CD73 a me ka pae ADA;2) hoʻonui ka hyperbaric hyperoxia i ke au;a ʻo 3) ʻaʻohe mana nui o ka hyperbaria wale nō i kēia mau ʻāpana.I loko o ka lolo a me ka cellulo, 1) ho'ēmi ka hyperoxia i ka'ōlelo A2AR;2) hoʻonui ka hyperbaric hyperoxia i ke au;a me 3) hyperbaria wale nō i hōʻike i ka hopena ikaika loa.Ua loaʻa iā mākou kahi ʻano like e pili ana i ka synthesis A2AR mRNA i loko o ka lolo a me ka hana ʻana o ka cAMP i nā ʻāpana Chem-3.No laila ua hoʻohaʻahaʻa ka pae oxygen kiʻekiʻe i ke ala adenosinergic a me ka hana CD26.ʻO ka Hyperbaria wale nō i hoʻopili wale i ka hōʻike A2AR a me ka hana cAMP.Kūkākūkā mākou pehea e hiki ai i nā hana i hoʻoulu ʻia e ka hyperoxygenation ke kaupalena, ma o ka vasoconstriction, ka hāʻawi ʻana o ka oxygen i nā ʻiʻo a me ka hana ʻana o nā ʻano oxygen reactive.