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Carbonic anhydrase activators: ʻo ka haʻawina hoʻāla mua o ka isoform VI huna huna kanaka me nā amino acids a me nā amine.

ʻO ka isozyme huna o ke kanaka carbonic anhydrase (hCA, EC 4.2.1.1), hCA VI, ua hoʻopili ʻia, hōʻike ʻia, a hoʻomaʻemaʻe ʻia i loko o kahi ʻōnaehana hōʻike bacterial.Ua hōʻike ʻia nā ʻāpana kinetic no ka hopena hydration CO(2) i ka hCA VI e loaʻa iā ak(cat) o 3.4 x 10(5)s(-1) a me k(cat)/K(M) o 4.9 x 10(7)M (-1)s(-1) (ma ka pH 7.5 a me 20 degere C).Loaʻa i ka hCA VI kahi hana catalytic koʻikoʻi no ka hopena physiological, e like me ka nui o ka isoform CA I a i ʻole ka transmembrane, isozyme CA IX pili i ka tumor.Ua hōʻike ʻia kahi pūʻulu o nā amino acids a me nā amine e hana ma ke ʻano he CA VI activators, me nā ʻano like ʻole.Ua hōʻike ʻo l-His, l-Trp, a me dopamine i nā hopena hoʻonāwaliwali CA VI (K (A) s i ka laulā o 21-42 microM), akā ʻo d-His, d-Phe, l-DOPA, l-Trp, serotonin. , a ʻo kekahi mau pyridyl-alkylamines he mau mea hoʻoikaika maikaʻi loa, me K (A) s i ka laulā o 13-19 microM.ʻO nā mea hoʻolale CA VI maikaʻi loa he l-Phe, d-DOPA, l-Tyr, 4-amino-l-Phe, a me ka histamine, me K (A) s i ka laulā o 1.23-9.31 microM.Hoʻonui kēia mau mea hoʻoulu i ka k(cat), ʻaʻohe hopena i ka K (M), e komo pū ana i ka pae hoʻoholo helu i ka pōʻaiapili catalytic, nā hopena hoʻololi proton ma waena o ka pae hana enzyme a me ke kaiapuni.