Nā huahana

Nā waiwai physicochemical ʻO ka wai hoʻoheheʻe o ka acridine a me kāna mau paʻakai e hōʻike ana i ka fluorescence poni a ʻōmaʻomaʻo paha.Loaʻa ka fluorescence ʻōmaʻomaʻo i nā hopena o nā paʻakai, a ke hoʻoheheʻe hou ʻia, ma muli o ka hydrolysis o nā paʻakai, lilo lākou i acridine manuahi, e hōʻike ana i ka fluorescence poni.He alkaline nawaliwali ka solution aqueous a hana aku me na waikona inorganic e hana i paakai.Paʻa loa ka Acridine, ua like kona ʻano me ka anthracene, a ua like loa kona mau waiwai kemika.ʻO ka mahu a me ka hopena he mea hoʻonāukiuki, hoʻonāukiuki nui i kaʻili a me ka mucous membranes, aʻo ka hoʻouluʻana i ka mahu e hiki ai ke hoʻoulu i ka maʻi.

Ma ke ʻano he luminescent probe, hoʻohana ʻia ia i ke aʻo ʻana i nā ʻāpana gene.Hoʻopili ʻia ka hopena me ka acridan (9,10-dihydroacridine) ma ke ʻano he substrate a me ka alkaline phosphatase, e hana ana i ka chemiluminescence kiʻekiʻe.Hāʻawi i ka naʻau maikaʻi a me ka maʻalahi o ka hoʻohana ʻana no ka conjugation alkaline phosphatase i ka wā ʻike chemiluminescent.ʻIke ʻia ka alkaline phosphatase ma lalo o 10-19 mol, piʻi wikiwiki e hōʻemi i ka manawa ʻike a hoʻonui i ka throughput, a ua hoʻolālā ʻia ka slope o ka linear calibration curve me ka logarithmic like me 1.0.Hoʻokahi nui a ʻoi aʻe paha o ka enzyme e hoʻopuka i hoʻokahi nui a ʻoi aʻe paha o ka luminescence, hoʻomau mau ka luminescence-ʻaʻole koi nui i ka manawa hoʻāʻo.Hiki ke heluhelu ʻia ka ikaika o ka luminescence mai ka ʻōkuhi linear calibration i hana ʻia i kēlā me kēia manawa, a ʻaʻole i ʻike nā hopena analytical i ka mahana ma waena o 22 ° C - 35 ° C, e hōʻemi ana i ka pololei e pono ai ke kāohi i ka mahana.

Noi: Hiki ke hoʻohana ʻia no ka hoʻopaʻa inoa ʻana i nā protein, antigens, antibodies, nucleic acids (DNA, RNA), etc.