Nā huahana

ʻO LecA (PA-IL) he lectin cytotoxic a me ka adhesin i hana ʻia e Pseudomonas aeruginosa e hoʻopaʻa ana i nā galactosides hydrophobic me ka kikoʻī kiʻekiʻe a me ka pili.Ma ka hoʻohana ʻana i ka unuhi unuhi lecA-egfp a me ka nānā ʻana i ka immunoblot o ka matrix extracellular biofilm, hōʻike mākou ua hōʻike ʻia ka lecA i nā pūnaewele ulu biofilm.Ma nā ho'āʻo biofilm static ma ka polystyrene a me ke kila kila, ua hoʻemi ʻia ka hohonu o ka biofilm a me ka uhi o ka ʻili e ka hoʻololi ʻana o ka lecA a hoʻonui ʻia i ka LecA-overproducing strain PAO-P47.ʻO ka uhi ʻana o ka biofilm e ka makua, PAO-P47 akā ʻaʻole ka lecA mutant ma nā coupons kila i kāohi ʻia e ka ulu ʻana i mua o ka isopropyl-beta-D-thiogalactoside (IPTG) a i ʻole p-nitrophenyl-alpha-D-galactoside ( NPG).Eia kekahi, hiki ke hoʻopuehu ʻia nā biofilms ʻano ʻāhiu makua i ka ʻole o kēia mau galactosides hydrophobic e ka hoʻohui ʻana o IPTG.I ka hoʻokaʻawale ʻana, ʻaʻohe hopena o ka hoʻohui ʻana o ka p-nitrophenyl-alpha-L-fucose (NPF) i loaʻa kahi pilina kiʻekiʻe no ka lecti n P. aeruginosa LecB (PA-IIL) i ka hoʻokumu ʻana i nā biofilm a i ʻole ka hoʻopuehu ʻana.ʻAʻole i hoʻopilikia ʻia ka ulu ʻana o ka planktonic o P. aeruginosa PAO1 i ka hiki ʻana mai o IPTG, NPG a i ʻole NPF, ʻaʻole hoʻi i hiki i ke kānana ke hoʻohana i kēia mau sugars ma ke ʻano he kumu kalapona, e hōʻike ana i nā hopena i ʻike ʻia ma ka hoʻokumu ʻana o biofilm ma muli o ka hoʻokūkū hoʻokūkū o LecA-ligand. paa.Ua loaʻa pū nā hopena like no nā biofilms i ulu ma lalo o nā kūlana kahe ikaika ma nā coupons kila, e hōʻike ana e kōkua ʻo LecA i ka P. aeruginosa biofilm architecture ma lalo o nā kūlana kaiapuni like ʻole.

ʻO kahi alpha-L-fucosidase (EC 3.2.1.51) hiki ke hoʻokuʻu i ke koena t-fucosyl mai ke kaulahao ʻaoʻao o xyloglucan oligosaccharides i ʻike ʻia i nā lau o nā mea kanu Arabidopsis.Eia kekahi, ua hoʻomaʻemaʻe ʻia kahi alpha-L-fucosidase me ke ʻano like o ka substrate mai nā lau kāpeti (Brassica oleracea) e hoʻolilo i hoʻokahi hui ma SDS-PAGE.Ua loaʻa mai ʻelua mau kaʻina peptide mai kēia pūnaʻi protein, a ua hoʻohana ʻia lākou e ʻike i kahi Arabidopsis gene coding no kahi alpha-fucosidase a mākou e manaʻo nei e kapa iā AtFXG1.Eia kekahi, ua loaʻa pū kekahi gene Arabidopsis me ka homology me nā alpha-L-fucosidases i ʻike ʻia, a ua manaʻo mākou e kapa iā ia ʻo AtFUC1.Ua hōʻike ʻia ʻo AtFXG1 a me ATFUC1 i heterologously i loko o Pichia pastoris cell a me nā hana alpha-L-fucosidase i hūnā ʻia i ka moʻomeheu moʻomeheu.ʻO ka alpha-L-fucosidase i hoʻopaʻa ʻia e AtFXG1 e kūʻē i nā oligosaccharides mai xyloglucan XXFG a me 2'-fucosyl-lactitol akā ʻaʻole kūʻē i ka p-nitrophenyl-alpha-L-fucopyranoside.Eia naʻe, ʻo ka AtFUC1 heterologously i hōʻike ʻia he ikaika wale nō i ka 2'-fucosyl-lactitol.No laila, pono e pili ka mea mua i ka metabolism xyloglucan.