Nā huahana

ʻO ka hoʻokaʻawale ʻana o nā fibroblasts orbital i nā adipocytes makua a me ka hōʻiliʻili ʻana o ka ʻiʻo adipose i hōʻike ʻia i ka holomua o ka Graves 'orbitopathy (GO).Hoʻokomo ʻia ʻo Autophagy i adipogenesis, akā ʻike liʻiliʻi e pili ana i ke kuleana o autophagy i ka hoʻomaka a me ka holomua o GO.ʻO ka pahuhopu o kēia haʻawina e noiʻi i ke kuleana o ka autophagy i ka pathogenesis o GO.Orbital adipose / connective tissue explants mai nā maʻi me GO a mai nā kumuhana maʻamau, a me nā fibroblast orbital kaʻawale, ua kālailai ʻia.Ua hoʻokomo ʻia ʻo Adipogenesis me ka hoʻohana ʻana i ke ʻano like ʻole me ka hydrogen peroxide a i ʻole ka hydrogen peroxide, a ua hoʻohana ʻia ka autophagy me ka hoʻohana ʻana i ka bafilomycin A1 a me ka Atg5-targeted short hairpin RNA (shRNA).Ua ʻike ʻia nā autophagosome e ka microscopy electron.ʻO ka hōʻike ʻana i nā genes pili i ka autophagy a me nā kumu transcription pili i adipogenesis i kālai ʻia e ka manawa maoli reverse transcription-polymerase chain reaction a / a i ʻole ka nānā ʻana o Western blot.Ua nānā ʻia ka hōʻiliʻili lipid droplet e ka Oil Red O staining. ʻOi aku ka nui o nā vacuoles Autophagic i loko o nā pūnaewele GO ma mua o nā pūnaewele non-GO (p<0.05).ʻOi aku ka kiʻekiʻe o ka hōʻike ʻana o nā genes pili autophagy i nā ʻiʻo a me nā cell GO ma mua o kā lākou mau hoa non-GO, kēlā me kēia.Hoʻonui ka Interleukin-1β i ka LC3-II, p62, a me ka protein Atg7 i loko o nā pūnaewele GO.Ua hōʻike ʻia ka hōʻiliʻili autophagosome i ka lā 4 o adipogenesis a ua hoʻemi ʻia e ka lā 10, me ka hoʻokumu ʻana o ka lipid droplet.Ua hoʻonui ʻia ka hōʻike ʻana o nā protein LC3 a me p62 i loko o 48 mau hola o ka hoʻokaʻawale ʻana a hoʻemi mālie ʻia mai ka lā 4 a hiki i ka 10. ʻO ka mālama ʻana ʻo Bafilomycin A1 a me ka Atg5 knockdown e shRNA ua kāohi i ka hōʻiliʻili ʻana o ka lipid droplet a me ke kāohi ʻana i ka hōʻike ʻana o nā māka adipogenic. non-GO kiko a me nā pūnaewele, e hōʻike ana he kuleana ko autophagy i ka pathogenesis GO.ʻO ka manipulation Autophagy paha he pahuhopu therapeutic no GO.