Nā huahana

Ke ʻimi nui ʻia nei nā pūhui multitarget no ka mālama pono ʻana i nā maʻi paʻakikī.Ma ʻaneʻi, wehewehe mākou i ka hoʻolālā a me ka synthesis o kahi papa hou o nā hybrids shogaol-huprine, i manaʻo ʻia e paʻi i kekahi mau pahuhopu nui i pili i ka maʻi o Alzheimer.Ua hoʻāʻo ʻia nā hybrids i loko o ka vitro no kā lākou hana pale e kūʻē i ka acetylcholinesterase kanaka a me ka butyrylcholinesterase a me ka hana antioxidant (ABTS.+, DPPH a me Folin-Ciocalteu assays), a i loko o nā pūnaewele Escherichia coli paʻa no kā lākou Aβ42 a me ka hana anti-aggregating.Eia kekahi, ua loiloi ʻia ko lākou komo ʻana o ka lolo (PAMPA-BBB assay).ʻOiai ʻaʻole like nā hybrids e like me ka AChE inhibitors a i ʻole nā ​​​​mea antioxidant e like me ka makua huprine Y a me [4]-shogaol, i kēlā me kēia, ke hōʻike mau nei lākou i ka ikaika anticholinesterase a me nā hana antioxidant a ʻoi aku ka ikaika o ka Aβ42 a me ka tau anti-aggregating agents ma mua o ka hui makua.Ma ke ʻano holoʻokoʻa, puka mai nā huila shogaol-huprine e like me ke alakaʻi anti-Alzheimer me ka lolo permeable multitarget.

Ua aʻo ʻia nā ʻāpana o nā polarity like ʻole mai nā ʻāpana mea kanu like ʻole (aʻa, lau, pua a me nā hua) o Seseli rigidum e nā ʻano hoʻokolohua antioxidant like ʻole: DPPH a me ABTS radical scavenging activity, ma ka hōʻemi ʻana i ka mana a me ka huina o nā flavonoids a me nā polyphenols. .ʻO nā aila pono o nā ʻāpana mea kanu i hōʻike i nā ʻano antioxidant nāwaliwali.He 0.01-1.50 mg/mL ka 0.01-1.50 mg/mL a me ka 0.01-1.50 mg/mL o ka microbicidal 0.02-3.00 mg o ka microbicidal cereus, Bacillus cereus, a me ka fungi Candida albicans a me Aspergillus niger ka laula hoʻopaʻa paʻa o nā mea i hoʻāʻo ʻia.I ka launa pū ʻana me ka cholinesterase, ua ʻike maikaʻi nā ʻaila koʻikoʻi āpau ma ke ʻano he inhibitors.Ua hōʻike ʻia ka pākēneka kiʻekiʻe loa o ka inhibition versus kanaka a me ka lio cholinesterase e ke kumu aila nui (38.20% a me 48.30%, kēlā me kēia) i waena o nā aila, a me ke kumu hexane extract (40.56% a me 50.65% pakahi).Ua ʻike ʻia nā ʻaila koʻikoʻi a me nā ʻāpana o nā mea kanu āpau e GC, GC-MS a me headspace/GC-MS.Ua hōʻike ʻia ka ʻikepili helu o ka hui o nā hopena i ka ʻokoʻa o ka hui ʻana o ka ʻaila koʻikoʻi mai nā ʻaila koʻikoʻi o nā ʻāpana ʻē aʻe o ka mea kanu.Ma ka noʻonoʻo ʻana i nā hana a pau i aʻo ʻia, ua hōʻike ʻia ke kumu hexane extract i nā waiwai holoʻokoʻa maikaʻi loa.Ma o ka chromatography wai hana kiʻekiʻe i hui pū ʻia me ka spectrometry nuipa hoʻonā kiʻekiʻe, ua ʻike ʻia ka 30 mea nui loa i nā unuhi o nā polarity like ʻole.Ua hoʻopili ʻia ka noho ʻana o nā mea i ʻike ʻia i nā hana ola kino i nānā ʻia, no laila ke koho ʻana i nā pūhui i hiki ke kuleana no kēlā me kēia hana i hōʻike ʻia.