Streptomycin sulfate CAS:3810-74-0 Ka pauka keʻokeʻo
| Helu Helu | XD90361 |
| inoa mea kūʻai | ʻO Streptomycin sulfate |
| CAS | 3810-74-0 |
| ʻĀpana Molekala | (C21H39N7O12)2·3H2SO4 |
| Kaumaha Molecular | 1457.39 |
| Nā kikoʻī mālama | 2 a 8 °C |
| Kānāwai Kūʻai Kūlike | 29412080 |
Huahana Huahana
| Papa | USP36/EP8.0 |
| pH | 4.5-7.0 |
| Nalo ma ka maloo | ≤7.0% |
| Hoʻāʻo | ≥720IU/MG |
| Sulfate | 18.0~ 21.5% |
| Ka nana aku | ʻO ka pauka keʻokeʻo a kokoke i keʻokeʻo, hiki ke hoʻoheheʻe ʻia i ka wai, aneane hiki ʻole ke hoʻoheheʻe ʻia i ka ethanol. |
| Methanol | ≥0.3% |
| ʻO Streptomycin B | ≤3.0% |
| Ho'āʻo kalakala | ≥90.0% |
| ʻAka Sulfuric | ≤1.0% |
| ʻO ka sodium sulphite maʻamau | ≤0.4% |
ʻOiai ʻaʻole maopopo ka etiology o ka degeneration disc intervertebral, hoʻokahi ala e pale aku ai i kēia kaʻina hana ke kāohi i ka apoptosis.Ma ka haʻawina o kēia manawa, ua noiʻi ʻia nā hopena anti-apoptotic o carboxymethylated chitosan (CMCS) i loko o nā pūnaewele nucleus pulposus (NP) me ka manaʻo e hoʻonui i ke ola o ka cell disc.Ua hoʻokaʻawale ʻia nā pūnaewele NP ʻiole a hoʻoulu ʻia i loko o ka vitro, a ua hoʻohana ʻia ka hydrogen peroxide (H2O2) no ke kūkulu ʻana i ke kumu hoʻohālike NP cell apoptosis.Ua loiloi ʻia ke ola o ke kelepona me kahi helu helu cell-8 assay.Ua noiʻi ʻia ka ratio o nā cell apoptotic e annexin V‑fluorescein isothiocyanate (FITC) a me propidium iodide (PI) ka nānā ʻana i ka ʻili pālua, a ua ʻike ʻia ka morphology e ka hoʻopaʻa ʻana o Hoechst 33342.Ua loiloi ʻia ka mana mitochondrial membrane o nā pūnaewele NP e ka rhodamine 123 fluorescence staining.Hana ʻia ka hoʻohuli ʻana (RT)‑quantitative polymerase chain reaction (qPCR) e ana i nā pae mRNA o inducible nitric oxide synthase (iNOS), caspase‑3, B‑cell lymphoma (Bcl)‑2, type II collagen a aggrecan.Ua hana ʻia ka loiloi Western blot e ʻike i nā pae protein o iNOS a me Bcl‑2.Ua hōʻike ʻia nā hopena staining annexin V‑FITC/PI a me Hoechst 33342 ua hiki i ka CMCS ke pale aku i nā pūnaewele NP mai ka apoptosis ma ke ʻano i hilinaʻi ʻia.Ua wehewehe ʻo Rhodamine 123 staining i ka hoʻemi ʻana o CMCS i ka hōʻino ʻana o ka hiki o ka membrane mitochondrial i nā cell NP i mālama ʻia e H2O2.Ua hōʻemi ʻia ka caspase-3 a me ka hoʻonui ʻana i ka hana Bcl-2 i loko o nā cell NP i mālama ʻia e CMCS e RT‑qPCR a me ka nānā ʻana o ke komohana blot.Hoʻolaha pū ʻo CMCS i ka hoʻonui ʻana a me ka huna ʻana o ke ʻano II collagen a aggrecan i loko o ka H2O2-treated NP cell.Ua hōʻike ʻia ʻo CMCS i mea maikaʻi i ka pale ʻana i ka make apoptotic cell in vitro, e hōʻike ana i nā pono kūpono o kēia ala therapeutic i ka hoʻoponopono ʻana i ka degeneration disc.
