SYBR GREEN I Nucleic Acid Gel Stain Cas: 163795-75-3 99% Solid
| Helu Helu | XD90236 |
| inoa mea kūʻai | SYBR GREEN I Nucleic Acid Gel Stain |
| CAS | 163795-75-3 |
| ʻĀpana Molekala | C32H37N4S |
| Kaumaha Molecular | 509.728 |
| Nā kikoʻī mālama | -20°C |
Huahana Huahana
| Ka nana aku | Pauda keʻokeʻo |
| Hoʻāʻo | 99% |
ʻO Flow cytometry kahi ʻano pahuhopu no ka hoʻokō ʻana i nā in vitro antimalarial sensitivity assays me ka hoʻonui ʻana i ka hiki ke noi ma nā kahua kahua.Ua nānā mākou in vitro susceptibility i ʻehiku mau lāʻau anti-malarial no 40 hou P. falciparum i hoʻokaʻawale ʻia ma o ka flow cytometry method (FCM), kahi colorimetric LDH-based ELISA: DELI), a me ka nānā ʻana i ka microscopic slide maʻamau o ka ulu.No FCM, 184/280 (66%) nā ho'āʻo i hoʻokō i nā koina ʻae analytical, hoʻohālikelike ʻia me 166/280 (59%) no DELI.Loaʻa ka ʻaelike maikaʻi ma waena o FCM a me ka microscopy, ʻoiai ʻo DELI ka mea e hana i ka hapalua-maximal inhibition constants (IC50s) ma mua o FCM, me ka hoʻohālikelike holoʻokoʻa o 2.2-fold (Bland-Altman hoʻohālikelike).ʻO nā waiwai no ka artesunate a me ka dihydroartemisinin i hoʻopilikia nui ʻia.ʻO ka piʻi ʻana o ka paradoxical i ka hōʻailona ma ke kiʻekiʻe kiʻekiʻe o ka mefloquine a me nā pūhui e pili ana i hōʻailona ʻia me ka DELI assay, e hōʻike ana he kuleana paha nā hopena ma waho o ka hana LDH.Ua ʻike ʻia ka nalo ʻana o ka hōʻailona FCM ma muli o ka hoʻouka hou ʻana a i ʻole ka ulu lohi ʻana i kahi helu liʻiliʻi o nā laʻana.Hoʻonui kēia mau hopena i ka hana mua e pili ana i ka hoʻohana ʻana i ka cytometry kahe e hoʻoholo ai i ka hiki ʻana o ka antimalarial ma ke ʻano o ka helu o nā laʻana, ka nui o nā lāʻau lapaʻau, a me ka hoʻohālikelike ʻana me nā ʻano hana ʻē aʻe.
