Tris maleate Cas: 72200-76-1 99.5% Keokeo crystalline pauda
| Helu Helu | XD90057 |
| inoa mea kūʻai | Tris maleate |
| CAS | 72200-76-1 |
| ʻĀpana Molekala | C4H11NO3 · C4H4O4 |
| Kaumaha Molecular | 237.21 |
| Nā kikoʻī mālama | Ambient |
| Kānāwai Kūʻai Kūlike | 29221985 |
Huahana Huahana
| Ka nana aku | ʻO ka pauka kristal keʻokeʻo |
| Hoʻāʻo | ≥ 99.5% |
Cocrystallization o lysyl-tRNA synthetase mai Thermus thermophilus me kona mau tRNAlys cognate a me Escherichia coli tRNAlys.
Ua hoʻopili ʻia ʻo Lysyl-tRNA synthetase mai Thermus thermophilus me kāna tRNAlys cognate a i ʻole Escherichia coli tRNAlys e hoʻohana ana i ka ammonium sulfate ma ke ʻano he precipitant.Ke ulu nei nā kristal mai nā hāʻina i loaʻa kahi stoichiometry 1:2.5 o ka synthetase dimer i tRNA ma 18-22% ammonium sulfate ma 50 mM Tris-maleate buffer ma pH 7.5.Hoʻokumu ʻia nā paʻakikī ʻelua i ka square prismatic, tetragonal crystals me nā ʻāpana cell unit like loa (a = b = 233 A, c = 119 A) a ʻokoʻa i ka liʻiliʻi he 2.7 A hoʻonā.Eia nō naʻe, ʻo ka homocomplex o ka pūʻulu ākea P42(1)2 a me ka heterocomplex o ka hui ākea I422.
Pani
