Nā huahana

1. Ua aʻo mākou i nā hopena pale o ka isorhamnetin ma ka mushroom tyrosinase e nā kinetics inhibition a me ka simulation computational.Isorhamnetin reversibly inhibited tyrosinase ma ke ano huikau ma Ki=0.235±0.013 mM.ʻO nā ana o ka intrinsic a me ka 1-anilinonaphthalene-8-sulfonate (ANS) -binding fluorescence i hōʻike i ka isorhamnetin ʻaʻole i hoʻohuli i nā loli koʻikoʻi i ke ʻano tertiary o tyrosinase.No ka loaʻa ʻana o ka ʻike i ke kaʻina hana inactivation, ua helu ʻia nā kinetics ma o nā ana manawa-manawa a me nā hopena substrate mau.ʻO nā hopena i hōʻike ʻia ʻo ka inactivation i hoʻokomo ʻia e ka isorhamnetin kahi hopena mua me nā kaʻina biphasic.No ka loaʻa ʻana o ka ʻike hou aku, ua hoʻohālikelike mākou i ka docking ma waena o tyrosinase a me isorhamnetin.Ua kūleʻa ka simulation (nā ikehu hoʻopaʻa no Dock6.3: -32.58 kcal/mol, no AutoDock4.2: -5.66 kcal/mol, a no Fred2.2: -48.86 kcal/mol), e hōʻike ana e pili ana ka isorhamnetin me kekahi mau koena, penei e like me HIS244 a me MET280.ʻO kēia hoʻolālā o ka pilina tyrosinase predict i hui pū ʻia me nā kinetics i hoʻokumu ʻia i kahi pūhui flavanone hiki ke hōʻoia i ka pono i ka nānā ʻana i nā mea hoʻopaneʻe tyrosinase kūlohelohe.

2. Acid unfolding ala o conalbumin (CA), he monomeric glycoprotein mai hen hua manu keokeo, ua noiʻi me ka mamao- a kokoke-UV CD spectroscopy, intrinsic fluorescence emission, extrinsic fluorescence probe 1-anilino-8-napthalene sulfonate (ANS) a me ka hoʻopuehu kukui ikaika (DLS).Nānā mākou i nā hoʻololi pili i ka pH i ke ʻano lua a me ke kula kiʻekiʻe o CA.Loaʻa iā ia he ʻano ʻano like ʻole α-helical lua ma ka pH 4.0 akā ʻo ka hana poho ma ka pH 3.0.Ua noho wale ka CA ma ke ano he pre-molten globule state a me molten globule state in solution ma pH 4.0 a me pH 3.0.ʻO ka hopena o ka pH i ka conformation a me ka thermostability o CA e kuhikuhi ana i kona pale ʻana i ka wela ma ka pH neutral.Hōʻike nā hualoaʻa DLS i ka noho ʻana o ka mokuʻāina ʻo MG ma ke ʻano paʻakikī i loko o nā wai wai me ka radii hydrodynamic o 4.7 nm.ʻO ke kinai ʻana o ka tryptophan fluorescence e ka acrylamide ua hōʻoia hou i ka hōʻiliʻili ʻana o kahi mokuʻāina waena, wehe ʻia kekahi hapa, ma waena o nā ʻāina ʻōiwi a wehe ʻole.