ʻO kahi moʻo β-glucosidase moʻolelo, bgl1G5, ua hoʻopili ʻia mai Phialophora sp.G5 a i hōʻike maikaʻi ʻia ma Pichia pastoris.Ua hōʻike ʻia ka hōʻike ʻana i ke kaʻina he 1,431-bp e hoʻopili ana i kahi protein o 476 amino acids.Ua hōʻike ʻia ke kaʻina amino acid o bgl1G5 i kahi ʻike kiʻekiʻe o 85 % me kahi β-glucosidase ʻano mai Humicola grsea o ka ʻohana glycoside hydrolase 1. Ke hoʻohālikelike ʻia me nā hoa fungal ʻē aʻe, ua hōʻike ʻo Bgl1G5 i ka hana maikaʻi loa ma ka pH 6.0 a me 50 °C a paʻa. ma ka pH 5.0–9.0.Eia kekahi, hōʻike ʻo Bgl1G5 i ka thermostability maikaʻi ma 50 °C (6 h half-life) a me ka hana kikoʻī kiʻekiʻe (54.9 U mg-1).ʻO nā koina K m a me V max i ka p-nitrophenyl β-D-glucopyranoside (pNPG) he 0.33 mM a me 103.1 μmol min-1 mg-1, kēlā me kēia.Ua hōʻike ʻia ka ʻike kikoʻī substrate he ikaika loa ʻo Bgl1G5 i ka pNPG, nāwaliwali ma ka p-nitrophenyl β-D-cellobioside (pNPC) a me ka p-nitrophenyl-β-D-galactopyranoside (ONPG), a ʻaʻohe hana ma cellobiose.
