He gene beta-glucosidase (bgl3) mai Streptomyces sp.QM-B814 (American Type Culture Collection 11238) ua hoʻopili ʻia e ka hoʻokō pono ʻana o kahi mutant beta-glucosidase-negative o Streptomyces lividans.Ua ʻike ʻia kahi papa heluhelu hāmama o 1440 nucleotides e hoʻopili ana i kahi polypeptide o 479 amino acid i loaʻa ma ke kaʻina hana.Hōʻike ka protein i hoʻopaʻa ʻia (Bgl3) i ka like nui (ma luna o 45% ʻike) me nā beta-glycosidases mai ka ʻohana-1 glycosyl hydrolases.ʻO ka enzyme cloned, i hoʻomaʻemaʻe ʻia ma hope o ka ua ʻana o ka ammonium sulphate a me nā ʻanuʻu chromatographic ʻelua, he monomeric me ka nuipaʻa mole 52.6 kDa, e like me ka hoʻoholo ʻana e ka spectrometry lehulehu, a me kahi kiko isoelectric o pI 4.4.ʻIke ʻia ka enzyme he beta-glucosidase me kahi kikoʻī o ka substrate ākea, e hana ana ma cellooligomers, a hana i nā hopena transglycosylation.ʻO nā helu Km i manaʻo ʻia no ka p-nitrophenyl-beta-D-glucopyranoside a me cellobiose he 0.27 mM a me 7.9 mM.ʻO nā waiwai Ki no ka glucose a me ka delta-gluconolactone, me ka hoʻohana ʻana i ka p-nitrophenyl-beta-D-glucopyranoside ma ke ʻano he substrate, he 65 mM a me 0.08 mM.ʻO ka enzyme i hoʻomaʻemaʻe ʻia he pH maikaʻi loa o ka pH 6.5 a ʻo ka mahana kūpono no ka hana he 50 degere
