D-Luciferin Cas: 2591-17-5 99% Keʻokeʻo i ka pauka melemele nbsp BEETLE LUCIFERIN
| Helu Helu | XD90248 |
| inoa mea kūʻai | D-Luciferin |
| CAS | 2591-17-5 |
| ʻĀpana Molekala | C11H8N2O3S2 |
| Kaumaha Molecular | 280.323 |
| Nā kikoʻī mālama | -15 a -20 °C |
| Kānāwai Kūʻai Kūlike | 29342080 |
Huahana Huahana
| Waiwai | Max.2.0% |
| ʻOhuehue | ʻO 2.0 NTU ka nui |
| ʻO ka hoʻololi ʻana i ka optical kikoʻī | -36 a hiki i -32 |
| Ka nana aku | Keʻokeʻo a i ka pauka melemele |
| HPLC maemae | Min 99% |
| ʻO ka helu hoʻopau mola | min 17900 L/(mol cm) |
Introduction: ʻO D-luciferin kahi pani no ka adenosine triphosphate (ATP) e pili ana i ka bioluminescence.ʻO ke kumumanaʻo o ka bioluminescence ʻo ia ka luciferin i hoʻokahe ʻia e luciferase i mua o ka ATP a me ka oxygen.Penei ke kumu hoʻohālikelike kemika: ATP+D-Luciferin+O2→Oxyluciferin+AMP+PPi+O2+Light.
Mechanism o ka hana: ʻO ke ʻano o ka hana o D-luciferin ma lalo o ka hana o ATP a me luciferase, luciferin (substrate) hiki ke oxidized e hoʻokuʻu i ka mālamalama.Ke ʻoi aku ka nui o ka luciferin, ua hoʻopili maikaʻi ʻia ka helu o nā photon i hana ʻia me ka ʻike o luciferase.
Noi: ʻO D-luciferin kahi pani no ka adenosine triphosphate (ATP) e pili ana i ka bioluminescence reactions.Hoʻohana pinepine ʻia ka hopena bioluminescence o luciferin/luciferase no ka ʻike ʻana i ka ATP, nā metabolites i hiki ke hoʻololi ʻia i ATP (e like me AMP, ADP, cAMP), a me nā enzymes hiki ke hoʻoulu i ka ATP (e like me ka creatine kinase, etc.), no laila. hiki ke hoʻohana ʻia ka hopena bioluminescence.Hoʻopili ʻia i ka ʻike ʻana i kahi ākea o nā mea olaola.
Nā Hana Ola: D-Luciferin (Firefly luciferin) he substrate kaulana ia no ka bioluminescence i mua o ka ATP no ka hoʻohanaʻana i ka luciferase-based bioluminescence imaging a me ka cell-based high-throughput screening.
Nā haʻawina in vitro: Hoʻopili ʻo D-luciferin me luciferase, ATP a me ka oxygen e hoʻokuʻu i ka mālamalama, i ʻike ʻia e ke kiʻi paʻi kiʻi maʻalahi e nānā i nā antibodies alkaline phosphatase-bound.
Ma nā haʻawina vivo: mālama ka hoʻohana ʻana i nā substrates D-luciferin a me firefly luciferase i ka launa pū ʻana o ka maʻi tumor-host i loko o kahi ʻano ʻiole immunocompetent me ka maʻi maʻi ovarian, no ka mea, ʻoi aku ka maʻalahi o nā papahana bioluminescence i ka ulu ʻana o ka maʻi ma mua o nā ʻōlelo aʻo e loaʻa ai ke kaumaha o ke kino.
