Nā huahana

No ka noiʻi ʻana i ke ʻano o ke koʻikoʻi oxidative a me / a i ʻole mitochondrial impairment i ka loaʻa ʻana o ka hōʻeha ʻeha maʻi (AKI) i ka wā sepsis, ua hoʻomohala mākou i kahi kumu hoʻohālike i hoʻokomo ʻia i loko o ka vitro me ka hoʻohana ʻana i nā cell epithelial tubular proximal i hōʻike ʻia i kahi endotoxin bacterial (lipopolysaccharide, LPS).Ua hāʻawi kēia noiʻi i nā hiʻohiʻona koʻikoʻi e pili ana i ka pilina ma waena o ke koʻikoʻi oxidative a me nā hemahema o ke kaulahao hanu mitochondrial.ʻO ka hoʻomaʻamaʻa LPS ka hopena i ka hoʻonui ʻana o ka inducible nitric oxide synthase (iNOS) a me NADPH oxidase 4 (NOX-4), e hōʻike ana i ka overexpression cytosolic o ka nitric oxide a me ka anion superoxide, nā ʻano mea hoʻoheheʻe mua (RNS) a me nā ʻano oxygen reactive. (ROS).Me he mea lā ua hoʻopau kēia mokuʻāina oxidant i ka mitochondrial oxidative phosphorylation ma ka hōʻemi ʻana i ka hana cytochrome c oxidase.Ma muli o ka hopena, ua hoʻopau ʻia ka lawe ʻana i ka electron a me ka proton pumping ma waena o ka membrane i loko o ka mitochondrial, e alakaʻi ana i ka emi ʻana o ka mitochond rial membrane potential, ka hoʻokuʻu ʻana i nā kumu hoʻoulu apoptotic a me ka pau ʻana o ka adenosine triphosphate.ʻO ka mea e mahalo ai, ma hope o ka manaʻo ʻia e RNS a me ROS, ua lilo ka mitochondria i mea hana o ROS, no laila e hāʻawi i ka hoʻonui ʻana i ka mitochondrial dysfunction.Ua hōʻoia hou ʻia ke kuleana o nā oxidants i ka mitochondrial dysfunction e ka hoʻohana ʻana i nā inhibitors iNOS a i ʻole antioxidants e mālama i ka hana cytochrome c oxidase a pale i ka hiki ʻana o ka mitochondrial membrane.Hōʻike kēia mau hopena i ka AKI i hoʻokomo ʻia i ka sepsis ʻaʻole pono e manaʻo ʻia he hemahema o ke kūlana o ka ikehu akā he pane hoʻohui pū kekahi, me nā hanana transcriptional, hōʻailona ROS, ka hana mitochondrial a me ka orientation metabolic e like me apoptosis.