Nā huahana

ʻO ka protein ORF45 o Kaposi's sarcoma-associated herpesvirus (KSHV) he gammaherpesvirus-specific immediate-early tegument protein.Ua hōʻike kā mākou mau haʻawina mua i kāna mau kuleana koʻikoʻi i ka wā mua a me ka hope o ka maʻi KSHV.Ma kēia haʻawina, ua nānā mākou i ka launa pū ʻana o ORF45 me ka hoʻohana ʻana i kahi papa o nā antibodies monoclonal.Ma kahi o ka extracellular regulated kinase (ERK) a me p90 ribosomal S6 kinase (RSK) proteins, ua loaʻa iā mākou kekahi mau protein copurified ʻē aʻe, me nā mea koʻikoʻi o ~38 kDa a me ~130 kDa.Ua hōʻike ʻia ka mass spectrometry ʻo ka protein 38-kDa he viral ORF33 a ʻo ka protein 130-kDa he cellular USP7 (ubiquitin-specific protease 7).Hoʻopili mākou i ka ORF33-binding domain i ka carboxyl-terminal 19 amino acids (aa) o ORF45 a me ka USP7-binding domain i ka motif consensus i hōʻike ʻia ma ke kikowaena o ORF45.Me ka hoʻohana ʻana i ka immunofluorescence staining, ʻike mākou i ka colocalization o ORF45 me ORF33 a i ʻole USP7 ma lalo o nā ion i hoʻololi ʻia a i loko o nā pūnaewele KSHV-infected.Eia kekahi, ua ʻike mākou i ka hoʻololi hou ʻana o ORF45 i kahi ʻāpana o ORF33/USP7 mai ka nucleus i ka cytoplasm.Ua ʻike mākou ua hoʻonui ʻo ORF45 i ka hōʻiliʻili protein ORF33 i hoʻopau ʻia inā i holoi ʻia ka ORF33- a i ʻole USP7-binding domain ma ORF45.Eia kekahi, ʻo ka holoi ʻana i ka pauku carboxyl i mālama ʻia o ORF45 i loko o ka genome KSHV i hoʻemi nui i ke kiʻekiʻe o ka protein ORF33 i loko o nā cell i hoʻopili ʻia e KSHV a hoʻopau i ka hana ʻana o nā virion progeny.ʻO ka hui pū ʻana, ʻaʻole hōʻike wale kā mākou hopena i nā mea hou o ka ORF45 interactome, akā hōʻike pū kekahi i ka pilina ma waena o kēia mau protein he mea koʻikoʻi no ka KSHV lytic replication. ʻO Kaposi's sarcoma-associated herpesvirus (KSHV) ke kumu kumu o kekahi mau maʻi maʻi kanaka.ʻO KSHV ORF45 he pūmua multifunctional i koi ʻia no ka hoʻopiʻi lytic KSHV, akā ʻaʻole maopopo nā ʻano hana pololei e hana ai ʻo ORF45 i kāna mau hana koʻikoʻi.Ua hōʻike ʻia kā mākou mau noiʻi mua ʻana aia nā mea āpau ORF45 i loko o nā cell i loko o nā complexes high-molecular-weight.No laila ua ʻimi mākou e hoʻohālikelike i ka launa pū ʻana o ORF45 e hāʻawi i nā ʻike i kāna mau kuleana i ka wā o ka lytic replication.Ke hoʻohana nei i kahi papa o nā antibodies monoclonal, ua nānā mākou i ka ORF45 interactome i loko o nā pūnaewele i hoʻopili ʻia e KSHV.Ua ʻike mākou i ʻelua mau hoa paʻa hou o ORF45: ʻo ka protein viral ORF33 a me ka protease ubiquitin-specific 7 (USP7).Ke hōʻike hou aku nei mākou he mea koʻikoʻi ka pilina ma waena o ORF45 a me ORF33 no ka hana maikaʻi ʻana o nā ʻāpana viral KSHV, e hōʻike ana i ka hoʻopili ʻia ʻana o kēia pilina e hōʻike i kahi hoʻolālā hou e kāohi ai i ka replication lytic KSHV.