Nā huahana

Ua hoʻohana maikaʻi ʻia ka chromatography kūʻē i kēia manawa i ka enantioseparation o dl-tryptophan me ka hoʻohana ʻana i ka bovine serum albumin ma ke ʻano he koho chiral.Ua hoʻohana ʻia kahi ʻōnaehana hoʻoheheʻe wai-wai biphasic 12.0% (w/w) polyethyleneglycol 8000-9.0% (w/w) dibasic potassium phosphate-0.1% ammonia-78.9% wai ma ke ʻano he ʻōnaehana solvent no ka chromatography counter-current, kahi i hoʻohana ʻia ai. ʻO ka bovine serum albumin i hoʻolaha nui ʻia ma ka pae haʻahaʻa o ka ʻōnaehana wai ʻelua.Hāʻawi ka ʻōnaehana hoʻoheheʻe wai-wai i kahi enantioselectivity kiʻekiʻe loa no ka d- a me ka l-tryptophan ma α=2.605 me ka lakene hoʻohele DD=1.200 a me DL=0.461.Loaʻa ka hoʻonā kiʻekiʻe kiʻekiʻe no ka enantioseparation o 2.0mg o dl-tryptophan e ka hui spiral tube counter-current chromatography ma lalo o ka mahana wela.Ua ʻike ʻia ʻo 0.1% amonia i hoʻohui ʻia i loko o ka ʻōnaehana hoʻoheheʻe wai-wai i hoʻomaikaʻi nui i nā enantioseparations.Ua ʻike ʻia kahi kiʻekiʻe ākea ākea loa no ka l-tryptophan i ka wā o nā antioseparations.No ka wehewehe ʻana, ua ana ʻia ka nui o ka hoʻololi ʻana o ka d- a me l-enantiomers ma o ke kikowaena ma waena o nā pae ʻelua.Ua ʻike ʻia ua hōʻike ʻo l-tryptophan i ka haʻahaʻa haʻahaʻa haʻahaʻa ma mua o d-tryptophan.Ua noi ʻia nā kūkākūkā hou aʻe no nā kumu kūpono no ka ʻokoʻa o ka hoʻololi nui ma waena o nā enantiomers.