Dithizone CAS: 60-10-6 80% ʻeleʻele uliuli pauka
| Helu Helu | XD90464 |
| inoa mea kūʻai | Dithizone |
| CAS | 60-10-6 |
| ʻĀpana Molekala | C13H12N4S |
| Kaumaha Molecular | 256.33 |
| Nā kikoʻī mālama | Ambient |
| Kānāwai Kūʻai Kūlike | 29309098 |
Huahana Huahana
| Nalo ma ka maloo | ≤5% |
| Ka nana aku | ʻeleʻele uliuli pauda |
| Hoʻāʻo | 80% |
| Koena ma ka Ignition | ≤0.2% |
Ua hōʻike ʻia ʻo Mesenchymal stem cell (MSCs) he kumu maikaʻi no ka hana ʻana o nā pūnaewele β surrogate transplantable.Ua ʻike ʻia kahi laina cell cell mesenchymal progenitor murine embryonic C3H10T1/2 ma ke ʻano he kumu hoʻohālike no nā MSC, ma muli o kona hiki ke hoʻokaʻawale ʻokoʻa.ʻO ke kumu o kēia noiʻi ʻana ʻo ia ka ʻimi ʻana inā he hiki ke hoʻokaʻawale ʻia nā cell C3H/10T1/2 i loko o nā cell hana insulin (IPCs).Maʻaneʻi, ua noiʻi mākou a hoʻohālikelike i ka hoʻokaʻawale in vitro o nā MSC iole a me C3H10T1 / 2 pūnaewele i loko o nā IPC.Ma hope o ka loaʻa ʻana o ka hoʻokaʻawale ʻana o ka IPC, ua ʻike ʻia ka hōʻike o nā māka hoʻokaʻawale e ka immunocytochemistry, reverse transcription-polymerase chain reaction (RT-PCR), quantitative real-time RT-PCR (qRT-PCR) a me Western blotting.Ua loiloi ʻia ka huna ʻana o ka insulin e ka enzyme-linked immunosorbent assay (ELISA).Eia kekahi, ua hoʻololi ʻia kēia mau ʻāpana ʻokoʻa i loko o nā ʻiole maʻi maʻi streptozotocin-induced a ua hoʻāʻo ʻia kā lākou mau hana olaola i vivo.Hōʻike kēia haʻawina i ke ʻano 2-pae e hoʻohua ai i nā IPC mai nā pūnaewele C3H10T1/2.Ma lalo o nā kūlana induction kiko'ī no nā lā 7-8, ua hoʻokumu nā pūnaewele C3H10T1 / 2 i nā kino spheroid ʻekolu-dimensional (SBs) a me ka insulin huna, ʻoiai ʻo ka hana ʻana o nā IPC i loaʻa mai nā MSC iole e koi i kahi manawa lōʻihi (ʻoi aku ma mua o 2 mau pule).Eia kekahi, ua hoʻokomo ʻia kēia mau IPC i loaʻa mai nā cell C3H10T1/2 i loko o nā ʻiole maʻi diabetic a hoʻomaikaʻi i ka glucose basal, ke kaumaha o ke kino a hōʻike i ka hoʻāʻo ʻana i ka glucose tolerance maʻamau.Hāʻawi ka haʻawina i kēia manawa i kahi hiʻohiʻona in vitro maʻalahi a kūpaʻa no ka noiʻi hou ʻana i ke ʻano o ka hoʻokaʻawale ʻana o IPC o nā MSC a me nā cell replacement therapy no ka maʻi diabetes.
