Nā huahana

He paʻakikī ka hopena o ka acidosis, kahi hōʻailona biochemical o ka microenvironment tumor, i ka piʻi ʻana o ka maʻi kanesa a me ka metastasis.Ua hōʻike ʻia nā hopena pro-a me anti-tumorigenic o ka acidosis a ua hoʻohana ʻia ka microenvironment acidic no ka lawe kikoʻī ʻana i nā lāʻau lapaʻau, nā mea hana kiʻi kiʻi, a me nā hana genetic i loko o nā ʻōpū.Ma kēia noiʻi ʻana mākou e noiʻi nei i ka laha ʻana a me ka hoʻopili ʻana o nā cell melanoma B16F10 i hana ʻia i ka genetically e hoʻomāhuahua i ka pH-sensing G protein-coupled receptor GPR4.Ma ka hoʻohana ʻana i ka hoʻopili ʻana i ka cell assays ua ʻike mākou ua hoʻopaneʻe ka overexpression GPR4 i ka hoʻolaha ʻana o ka cell a hoʻololi i ka localization spatial o ka dynamic focal adhesion complex, e like me ka localization o phosphorylated focal adhesion kinase (FAK) a me ka paxillin, ma ka pH acidic.Ua noiʻi pū ʻia ka G-protein a me nā ala hōʻailona o lalo i kuleana no kēia mau hopena.Ma ka hoʻohana ʻana i ka Rho inhibitor CT04 (C3 transferase), ka Rho-associated kinase (ROCK) inhibitors Y27632 a me thiazovivin, ka myosin light chain kinase (MLCK) inhibitor staurosporine a i ʻole kahi G12/13 inhibitory construct, cell spreading i hoʻihoʻi ʻia ʻoiai ka inhibition a ʻaʻole liʻiliʻi a ʻaʻohe hopena o ka hoʻāla ʻana o nā ala Gq a me Gs.Hōʻike ʻia kā mākou mau hopena ma o ke ala hōʻailona G12/13/Rho GPR4 e hoʻololi i ka dynamics adhesion focal a hōʻemi i ka hoʻolaha ʻana o ke kelepona a me ka hoʻoneʻe ʻana o ka membrane.