Nā huahana

ʻO kahi hopena ʻaoʻao i manaʻo ʻole ʻia o kekahi mau papa anticoagulants, ʻo ia ka osteoporosis a ma kahi liʻiliʻi paha, pili i ka hana deranged mesenchymal stem cell (MSC).ʻO ka pahuhopu o kēia haʻawina ʻo ia ka hoʻohālikelike ʻana i ka hopena o ka fondaparinux (FDP), kahi antithrombotic hou me kahi heparin kaumaha haʻahaʻa haʻahaʻa haʻahaʻa i hoʻohana nui ʻia, tinzaparin (TZP) ma ka MSC proliferation a me ka ʻokoʻa.Ua hoʻokaʻawale ʻia nā MSC mai ka iwi trabecular o nā maʻi trauma 14 e kahi kaʻina hana digestion e pili ana i ka collagenase a hoʻonui ʻia i nā kūlana maʻamau a hiki i ka paukū 3. ʻO ka hoʻonui ʻana a me ka hoʻokaʻawale ʻana o nā MSC i nā chondrocytes a me nā osteoblasts i loiloi ʻia me ka hoʻohui ʻole o FDP a me TZP me ka hoʻohana ʻana i ka maʻamau in vitro nā ho'āʻo a me kahi ākea o ka nui o ka lāʻau.Ua hoʻohana ʻia ka Flow cytometry no ka MSC phenotyping.Ma ka pūʻulu makahiki i aʻo ʻia (17-74 mau makahiki) ʻo ka pinepine MSC i nā hakina i hoʻokuʻu ʻia i collagenase he 641/10(6) mau pūnaewele (ma waena o 110-2,158) a ʻo ko lākou ulu ʻana he ∼ 4 mau lā / pālua ka lehulehu.Ua loaʻa i nā moʻomeheu kahi phenotype MSC maʻamau (CD73+, CD105+, CD146+, CD106+, a me CD166+).Ua loiloi ʻia ka hoʻonui ʻana o ke kelepona e nā colony-forming unit-fibroblast (CFU-F) a me colorimetric tetrazolium salt XTT assays.Ma nā ho'āʻoʻelua, ua kāohiʻia ka hoʻonuiʻana o MSC e ka hoʻohuiʻana o TZP, i nā mea kiʻekiʻe.Ma ka hoʻohālikelike, ʻaʻohe hopena o FDP i ka hoʻonui ʻana o MSC.ʻAʻole i hoʻopili ʻia ka ʻokoʻa Osteogenic a me ka ʻokoʻa chondrogenic e ka hoʻohui ʻana o TZP a i ʻole FDP.ʻOiai ʻo ka hoʻonui ʻana o MSC, akā ʻaʻole ka hoʻokaʻawale ʻana, ua hoʻopilikia maikaʻi ʻia e TZP, ʻaʻohe mea hōʻike no nā hopena ʻino o FDP i kēia ʻōnaehana hoʻohālike in vitro e hoʻopaʻapaʻa maikaʻi ana no kona hoʻohana ʻana i ka hoʻonohonoho orthopedic.