Nā huahana

Ua hoʻohana ʻia ka spectroscopy infrared e palapala i nā pilina substrate-protein: nā hoʻololi conformational o ka sarcoplasmic reticulum Ca(2+)-ATPase ma luna o ka nucleotide binding a me ka ATPase phosphorylation i nānā ʻia me ka hoʻohana ʻana i ka substrate ATP a me ATP analogues (2'-deoxy-ATP, 3 '-deoxy-ATP, a me ka inosine 5'-triphosphate), i hoʻololi ʻia ma nā pūʻulu hana kūikawā o ka substrate.ʻO nā hoʻololi i ka 2'-OH, ka 3'-OH, a me ka pūʻulu amino o adenine e hoʻemi i ka nui o ka hoʻololi conformational i hoʻopaʻa ʻia o ka ATPase, me nā hopena ikaika loa i ʻike ʻia no nā mea ʻelua.Hōʻike kēia i ka naʻau o ka nucleotide-ATPase complex i nā pilina pilikino ma waena o ka nucleotide a me ATPase.He mea koʻikoʻi nā hui a pau i aʻo ʻia no ka hoʻopaʻa ʻana a me ka launa pū ʻana o kahi hui ligand i hāʻawi ʻia me ka ATPase e hilinaʻi ʻia i nā pilina o nā hui ligand ʻē aʻe.Ua nānā ʻia ka Phosphorylation o ka ATPase no ITP a me 2'-deoxy-ATP, akā ʻaʻole no 3'-deoxy-ATP.ʻAʻohe loulou pololei ma waena o ka nui o ka hoʻololi conformational ma luna o ka paʻa nucleotide a me ka helu o ka phosphorylation e hōʻike ana ʻaʻole pono ka nui o ka hoʻololi conformational i hoʻokomo ʻia e ATP no ka phosphorylation.E like me ka nānā ʻana no ka paʻakikī nucleotide-ATPase, pili pū ka conformation o ka ATPase intermediate E1PCa(2) phosphorylated mua i ka nucleotide, e hōʻike ana he ʻano ʻano like ʻole o nā mokuʻāina ATPase ma mua o ka mea i manaʻo mua ʻia.