Nā huahana

Ma ke ʻano kinetic angiotensin-converting enzyme (ACE), he 80 mmol/L borate buffer ma ka pH 8.2 (37 degere C).ʻIke ʻia kahi pae lag i ka hopena, a manaʻo ʻia kahi incubation 5-min o ka substrate a me ka plasma ma mua o ke ana kinetic.ʻO ka substrate, N-[3-(2-furyl)acryloyl]-L-phenylalanylglycylglycine (FAPGG), ua hoʻonui ʻia ka manaʻo ma 1.0 mmol/L a ʻo ka lōʻihi o ke ana ma 345 nm e hōʻoia i ka linearity o ke ana.Hoʻohana ʻia ke kaʻina hana i ka 1:9 plasma-to-reagent volume ratio.Hoʻonui ka laina laina o ka assay ma kahi o 170 U/L, e hōʻike ana i kahi 25% substrate hydrolysis.Hoʻoholo ʻia ka absorptivity FAPGG ma ke ana ʻana i ka ʻokoʻa o ka absorbance ma waena o 1.0 mmol/L FAPGG a me nā hoʻonā huahana.Hoʻopaʻa ʻia ka ʻoiaʻiʻo o ka nalu ma ka ʻike ʻana i ka waiwai absorbance i manaʻo ʻia o ka solution FAPGG, a he 1.0-nm deviation mai 345 nm e hoʻololi i ka absorbance e 15.5%.ʻO ka pololei o ka ACE assays ma kahi o 60 a me 100 U/L ʻo 3.5% a me 2.4% i loko o ka pūʻulu a me 2.9% a me 2.6% ma waena o ka pūʻulu.He 41-139 U/L ka manawa kuhikuhi (2.5th a 97.5th percentiles), a ʻaʻohe ʻokoʻa ma waena o nā waiwai no nā kāne a me nā wahine.