Nā huahana

ʻO Acridine ester (NSP-SA-NHS) Cas199293-83-9 a me kona mau pūhui pili he mau lepili chemiluminescent maikaʻi loa nona ka paʻa, ka hana a me ka naʻau ma mua o kekahi mau radioisotopes.Hiki i ka Acridine esters ke hana me nā pūmua i loaʻa nā pūʻulu amino mua.Ma lalo o nā kūlana maʻamau, ua hoʻololi ʻia ʻo NHS ma ke ʻano he hui haʻalele, a ua hana ka protein i kahi paʻa amide paʻa me ka acridine ester.Ma hope o ka pau ʻana o ka hopena, ua hoʻoneʻe ʻia ka paʻakai acridinium nui ma o ke kolamu desalting.

ʻAʻole pono nā protein i kapa ʻia ʻo Acridine i ka catalysis enzymatic e hoʻopuka i ka mālamalama i mua o ka hydrogen peroxide alkaline.ʻO ke kumu kumu hoʻomāmā māmā, ʻo ia i loko o kahi solution hydrogen peroxide alkaline, ua hoʻouka ʻia ka acridine ester e nā iona hydrogen peroxide e hoʻohua i kahi dioxyethane paʻa ʻole me ka ʻāʻī, i hoʻoheheʻe ʻia i CO2 a me ka acridone hauʻoli uila.Ke hoʻi ka acridone i ka ʻāina, hoʻopuka ia i nā kiʻi me ka lōʻihi o ka hawewe absorption o 430 nm.He pōkole loa kēia kaʻina hana luminescence (ʻoi aku ka liʻiliʻi o ke kaʻina holoʻokoʻa ma mua o 2 kekona), a pono e hoʻohui ke kumu hoʻāla i kahi kiʻi kiʻi kūloko a me ka ʻike kiʻi kiʻi;Eia kekahi, hiki i kēia huahana ke hoʻohana i kahi mea heluhelu microplate multi-function i lako me kahi autosampler no ka hōʻiliʻili ʻike luminescence.Hiki ke hoʻopaʻa inoa ʻia nā protein, peptides, antibodies, a me nā waikawa nucleic me kēia huahana.Hoʻopuka wikiwiki nā esters Acridine i ka māmā ma lalo o ka hauʻoli o ka hydrogen peroxide alkaline, no laila hiki ke ʻike ʻia nā pūhui i hōʻailona ʻia ma ka ʻohi ʻana i nā kiʻi.

Hoʻohana nui ʻia kēia huahana no: chemiluminescence a me immunoassay, ka nānā ʻana i ka mea hoʻokipa, ka waikawa nucleic a me ka ʻike peptide a me nā noiʻi ʻē aʻe.