PMSF Cas: 329-98-6 98.0% keokeo crystalline pauda Phenylmethanesulfonyl fluoride (PMSF)
| Helu Helu | XD90250 |
| inoa mea kūʻai | Phenylmethanesulfonyl fluoride (PMSF) |
| CAS | 329-98-6 |
| ʻĀpana Molekala | C7H7FO2S |
| Kaumaha Molecular | 174.1927 |
| Nā kikoʻī mālama | Ambient |
| Kānāwai Kūʻai Kūlike | 29049900 |
Huahana Huahana
| Hoʻāʻo | ≥98.0% HPLC |
| Ka nana aku | pauda kristal keʻokeʻo |
ʻO ka PMSF he mea hiki ʻole ke hoʻololi i ka serine / cysteine protease inhibitor i hoʻohana mau ʻia i ka hoʻomākaukau ʻana i nā lysates cell.
Nā haʻawina in vitro: PMSF (2 mM) inhibited carbachol-stimulated inositol phosphate accumulation e 15% -19% wale nō i mua o Li +.ʻO ka pale ʻana i ka huli ʻana o ka phosphoinositide e ka PMSF ma muli o hoʻokahi a ʻoi aku paha nā ʻanuʻu ma hope o ka haki ʻana o nā phosphoinositides [1].PMSF inhibits acylation of inositol residues of GPI intermediates in the bloodstream of T. brucei.Kāohi ʻo PMSF i ka hoʻokumu ʻana o ka glycolipid C akā ʻaʻole ka hoʻoponopono hou ʻana o ka waikawa momona i loko o ka vitro.Kāohi ʻo PMSF i ka hoʻohui ʻana o ka GPI acylation a me ka ethanolamine phosphatase i loko o nā trypanosomes procyclic, akā ʻaʻole i nā cell Hela [2].
Ma nā haʻawina vivo: PMSF (0.1 mL/10 g b.wt, ip) i hana i ka antinociception, e like me ka mea i hōʻike ʻia e ka piʻi ʻana o ka dose-responsive i ka %MPE i ka loiloi latency tail-flick, akā ʻaʻole i hoʻopuka i ka hoʻopaʻa ʻana i ka lāʻau.ʻO nā ʻiole i loaʻa i kahi intraperitoneal injection o PMSF hōʻike i nā hopena cannabinoid me ka antinociception, hypothermia a me ka immobility me nā waiwai ED50 o 86, 224 a me 206 mg / kg, kēlā me kēia.Hoʻonui ka PMSF (30 mg / kg) pretreatment i nā hopena o ka anandamide ma nā pane tail-flick (antinociception), ka hana locomotor a me ka neʻe ʻana e 5-fold, 10-fold a me 8-fold, i kēlā me kēia [3].
Nā hoʻokolohua holoholona: Ua hoʻohana ʻia nā ʻiole kāne ICR ma ke kaupaona ʻana he 18 a 25 g i ka assay.Ua hoʻoheheʻe ʻia ʻo PMSF i ka aila sesame a lawelawe ʻia intraperitoneally i ka nui o 0.1 mL / 10 g b.wt.E lawelawe mau i ka PMSF 10 mau minuke ma mua o ka intravenous anandamide a i ʻole ka hoʻokele kaʻa.Ua hoʻomaʻamaʻa ʻia nā ʻiole i ka lumi loiloi i ka pō me ka ʻole o ka ʻai a me ka wai.Ma hope o ka anandamide intravenous a i ʻole ka hoʻokele kaʻa, ua loiloi ʻia kēlā me kēia holoholona penei: 5 mau minuke no ka tail-flick latency (antinociceptive) pane a me 5 a 15 mau minuke no ka hana kuʻuna (motor);a i ʻole 5 mau minuke no ka wela nui (rectal) a me ke apo Immobilization (catalepsy) no 5 a 10 mau minuke.
