Nā huahana

Ua hoʻomohala ʻia kahi hoʻāʻo wahi maʻalahi, e hiki ai ke helu i ka microalbuminuria.Ua hoʻokō ʻia ka loiloi e like me nā alakaʻi ISO 15189. Ua ʻike ʻia ka mimi ma nā ʻāpana cellulose acetate a hoʻopaʻa ʻia me ka hoʻohana ʻana i nā mea hoʻopaʻa paʻa protein maʻalahi (nigrosin, Coomassie Blue R-250, amido ʻeleʻele).Ua helu ʻia ka ikaika o ka waihoʻoluʻu o nā wahi ʻeleʻele me ka hoʻohana ʻana i kahi kikowaena Kodak Image 450. Ua ʻoi aku ka maikaʻi o ka nānā ʻana o ke ʻano kumu Coomassie Blue (18 mg/L) ma mua o ka nigrosin (50 mg/L) a i ʻole ʻeleʻele amido (100 mg/L). ) kumu hana.Ma loko o ka holo ʻana o ka hoʻololi (CV) a ma waena o ka holo CV o ka Coomassie blue assay, he 8.4% a me 9.7% (50 mg/L), a me 3% a me 4.5% (400 mg/L).No ka nigrosin, ʻo kēia mau ʻikepili, he 8.4 a me 9.4 (50 mg / L), a me 3.4 a me 6.4% (400 mg / L).Ua hōʻike ʻo Coomassie Blue i kahi koho paʻa paʻa i ka albumin.Ua ʻike ʻia ka laina laina ma waena o 20 a me 600 mg/L.Loaʻa ka pilina maikaʻi (r2 = 0.89) ma waena o Coomassie Blue a me nā ana immunonephelometric.Hiki ke ʻike ʻia ka albumin immuno-unreactive (i hoʻomākaukau ʻia e ka mālama ʻana i ka protease) e ka hoʻāʻo ʻokoʻa, e hāʻawi ana i kahi pono o ke ʻano me nā hoʻokolohua immunochemical.Hiki ke hoʻonui ʻia ka ua o ka Ammonium sulfate i ka kikoʻī o ka assay ma ka hoʻopau ʻana i nā hopena o nā kaulahao māmā manuahi.