Nā huahana

I mea e wehewehe ai i ke kaʻina hana mua o ka mineralization dentin, ua noiʻi ʻia ka hopena inhibitory o 1-hydroxyethylidene-1,1-bisphosphonate (HEBP) ma ka mineralization dentin.Hoʻokomo ʻia nā ʻiole (100 g) subcutaneously me HEBP (8 mg P/kg) no 7 a i ʻole 14 mau lā, a ua hana ʻia nā incisors no Ca histochemistry a / a i ʻole electron microscopy.Ua hōʻike ʻia nā ʻoki ʻoki i mālama ʻia e HEBP i nā lālani ʻē aʻe e like me ke alapiʻi o ka dentin mineralized a me ka non-mineralized ma ka hope apical.Hōʻike ʻo GBHA i nā hopena Ca maʻalahi i loko o ka matrix dentin circumpulpal non-mineralized kahi i hōʻike ʻia ai ka microscopy electron i ka hāʻawi ʻana i nā mea mesh-like electron-dense mesh.ʻAʻole i hoʻohuihui ʻia ka mānele dentin matrix no Ca akā he nui nā matrix vesicles (MVs) i hoʻopiha ʻia me nā waihona mineral crystalline a/a i ʻole amorphous.ʻO ka mineralization o ka circumpulpal dentin i loaʻa kūʻokoʻa i ka MV-rich mantle dentin layer i nā mea hoʻohālikelike.Hāʻawi kā mākou ʻikepili i nā hōʻike histochemical o ka waiwai hoʻopaʻa Ca-binding o ka circumpulpal dentin matrix a me kona nele i ka dentin mantle kahi i loaʻa ai ka mineralization-mediated MV.I loko o ka mantle dentin, ʻaʻole hoʻopilikia ʻo HEBP i ka ulu ʻana o ke aniani i MV akā pale i kona ulu ʻana ma hope o ka haki ʻana o ka membrane.Manaʻo ʻia e hiki i ka circumpulpal dentin matrix ke minerale kūʻokoʻa me ka MV-mediated mineralization o ka mantle dentin, ʻoiai nā MV e hoʻoholo i ka pae mua a me ka manawa o ka mineralization dentin.